Nanoscale structural dynamics of cell edge in breast tumour cells

What is it about?

We detailed nanoscale structural dynamics of filopodia, lamellipodia, cytoskeleton filament, microvilli and microbridge at cell edge in breast tumour cells revealed by scanning ion conductance microscopy.

Featured Image

Why is it important?

Cell migration plays a critical role in biological processes such as embryonic development, wound healing, cancer metastasis, and immune response. While molecular mechanisms regulating cell movement are well-studied, bridging the gap between these mechanisms and macroscopic cell behaviour remains a significant challenge due to the disparity in scale. At the subcellular level, an intermediate scale between molecular and cellular scales, cell membranes exhibit complex structural dynamics that are difficult to quantify and poorly understood. In this study, we utilized time-lapse scanning ion conductance microscopy to visualize subcellular nanoscale structural dynamics at the edges of breast cancer cells. Through quantitative analysis, we successfully identified three key features: (1) dynamic edges with abundant filopodia, (2) an inverse relationship between local cell migration rate and lamellipodia thickness, and (3) changes in the length and distance between cytoskeleton-filament-related structures following a Poisson process. These findings provide new insights into cell migration dynamics and contribute to bridging the gap between macroscopic and microscopic cellular motion.

Perspectives