Monitoring ATP hydrolysis and ATPase inhibitor screening using1H NMR

What is it about?

The publication describes a simple novel NMR-based method to monitor ATP or GTP hydrolysis. The method can be implemented as a medium throughput assay to identify kinase/ATPase inhibitors.

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Why is it important?

The method described in this publication, differently from existing methods, allows monitoring of ATP or GTP hydrolysis in an assay format that does not require any secondary reagents. Within an inhibitor screening effort the result is a lower risk of interference and false positives. The assay does not require specialized reagents and can be executed on a routine 500 MHz NMR spectrometer.