What is it about?
Previously the multi-color is popularly used for labeling of different subcellular organelles. Here we inversely applied the multiplexing, to attain fast and high fidelity super-resolution.
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Why is it important?
This technique can dramatically increase the imaging speed and fidelity for SOFI super-resolution. With Joint-tagging SOFI, we have obtained 85 nm resolution with imaging rate of 3s per frame, with much higher labeling density than conventional SOFI.
Perspectives
In Eric Betzig's 1995 OL paper which lead him to Nobel Prize, he has envisioned that through separation of the molecules in different "domains", one can achieve super-resolution. Here, by using the spectral differentiation, JT-SOFI has dramatically improved the performance of SOFI in temporal resolution and fidelity.
Dr Peng Xi
Peking University
Read the Original
This page is a summary of: Fast Super-Resolution Imaging with Ultra-High Labeling Density Achieved by Joint Tagging Super-Resolution Optical Fluctuation Imaging, Scientific Reports, February 2015, Nature,
DOI: 10.1038/srep08359.
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