What is it about?
The RRDY, RL and DPF were the top three out of twenty-one peptides for inhibitions against dipeptidyl peptidase-IV (DPP-IV) from the pepsin hydrolysis of yam dioscorin in silico, and were further to investigate the proof-of-concept study in normal ICR mice for regulating glucose metabolism by the oral glucose tolerance test (OGTT). The sample or sitagliptin (positive control) was orally administered by the feeding gauge, 30 min later, the glucose loads (2.5 g/kg) were performed.
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Why is it important?
The RRDY, yam dioscorin or sitagliptin preload, but not DPF, lowered area under curve (AUC0-120) of blood glucose and DPP-IV activity, and elevated AUC0-120 of blood insulin which showed differences significantly compared to those in the control (P < 0.05 or 0.001). These results suggested that RRDY and yam dioscorin might be beneficial in glycemic controls in normal mice and need further investigations in diabetic animal models.
Perspectives
the current results demonstrated that the proof-of-concept study in normal ICR mice of RRDY and RL from in vitro DPP-IV inhibitory activity screenings showed improved glucose metabolism by OGTT, and RRDY preload at a dose of 100 mg/kg showed sustained higher levels of lasting blood insulin and in turn reduced postprandial blood glucose levels compared to the blank after administration of the glucose load (2.5g/kg). The purified yam dioscorin preload was also shown to reduce postprandial blood glucose levels after the glucose load. These results suggested that the ingested RRDY and yam dioscorin were potential glycemic controls in normal mice in part by DPP-IV inhibitions. The yam dioscorin or RRDY peptide might be beneficial to developing healthy (functional) foods for the improvement of glucose intolerance and/or T2DM and need further investigations in diabetic animal models.
Professor Wen-Chi Hou
Taipei Medical University
Read the Original
This page is a summary of: Synthesized Peptides from Yam Dioscorin Hydrolysis in Silico Exhibit Dipeptidyl Peptidase-IV Inhibitory Activities and Oral Glucose Tolerance Improvements in Normal Mice, Journal of Agricultural and Food Chemistry, August 2016, American Chemical Society (ACS),
DOI: 10.1021/acs.jafc.6b02403.
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