What is it about?
This method has a powerful detection unit, based on thermal lens spectroscopy. Thus, it can analyze both bilirubin and biliverdin in biological samples at very low concentration. This means that the sample preparation is limited, which preserves the molecules to analyze.
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Why is it important?
This method has enabled the first-ever detection of biliverdin in human serum (ca. 2 nM) and first-ever assessement of bilirubin (free)/biliverdin ratio in human serum (ca. 2-4).
Perspectives
We are ready to measure bilirubin (free)/biliverdin ratio in human serum for clinical research.
Professor Sabina Passamonti
University of Trieste
Read the Original
This page is a summary of: Application of high-performance liquid chromatography combined with ultra-sensitive thermal lens spectrometric detection for simultaneous biliverdin and bilirubin assessment at trace levels in human serum, Talanta, July 2016, Elsevier,
DOI: 10.1016/j.talanta.2016.03.053.
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