What is it about?
The article reports the development of the CRISPR/Cas9-based genome editing method in Pseudomonas aeruginosa and cytidine deaminase-mediated base editing methods in Pseudomonas species, which enable highly efficient, rapid, and scarless genetic manipulations, including gene deletion, insertion, point mutations, and gene inactivation.
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Why is it important?
The methods will dramatically accelerate the genome engineering of Pseudomonas species.
Read the Original
This page is a summary of: CRISPR/Cas9-based Genome Editing in Pseudomonas aeruginosa and Cytidine Deaminase-Mediated Base Editing in Pseudomonas Species, iScience, August 2018, Elsevier,
DOI: 10.1016/j.isci.2018.07.024.
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