Experimental and theoretical study of GOS formation in CRMR by thermostable mesophilic enzymes

What is it about?

Galactosyl–Oligosaccharides (GOS) can be produced from lactose by enzymatic catalysis with b- galactosidases. GOS can be produced with free enzymes compartmentalized in continuous recycle membrane reactors (CRMR), or with scaffold-immobilized enzymes in plug flow bed reactors (PFBR). For describing GOS production from lactose in a CRMR, rigorous modelling yields complex enzymatic kinetic equations with a large number of constants. Unfortunately, such models are unhandy and are not convenient for practical purposes.

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Why is it important?

In agreement with the experiment outcome, the model predicted that the two CRMR in series produces higher GOS than the single CRMR. Although, compared with PFBR, was obtained the same GOS productivity but higher GOS productivity that obtained in other PFBR running reported in Ref. [2]. No enzyme activity leaked into the permeate.

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