Inhibitory effect of gold nanoparticles conjugated with interferon gamma and methionine on breast cancer cell line

Nastaran Mohseni, Fatemeh Salehi Sarvestani, Mehdi Shafiee Ardestani, Fatemeh Kazemi-Lomedasht, Masoud Ghorbani
  • Asian Pacific Journal of Tropical Biomedicine, February 2016, Elsevier
  • DOI: 10.1016/j.apjtb.2015.10.014

Gold nanoparticles conjugated with interferon gamma and methionine could reduce tumor cells

What is it about?

Gold nanorods (10 nm) were conjugated with IFN-γ and methionine using carbodiimide family and characterized after purification by dialysis bags. Breast cancer cells were cultured and incubated with gold nanorods at different concentrations followed by irradiation with the near-infrared laser beam. Samples were then evaluated for their viability in order to determine the effect of treatment and variables by MTT assay. Results: Zetasizer results confirmed the conjugation of gold nanorods with methionine and IFN-γ. The median percentage of cell viability in 0.30 μg/mL concentration of gold nanorods was 82%. The cell viability reached to 85% at the same concentration of gold nanorods, which existed in the assayed complex. The results of MTT assay showed that the 0.64 μg/ mL concentration of gold nanoparticles complex was toxic on tumor cells (P < 0.05). After exposure to hyperthermia, the viability of cells at 6 min decreased to 77% in 0.30 μg/mL of gold nanorods complex.

Why is it important?

The size and concentration of gold nanorods were not cytotoxic and during the irradiation with near-infrared laser, it increased the number of dead cells during the treatment of cells.


Dr Masoud Ghorbani
Pasteur Institute of Iran

I believe this publication could be useful for those who are working in the field of cancer treatment using nano drugs and nano complexes.

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The following have contributed to this page: Dr Masoud Ghorbani and Dr Masoud Ghorbani