A convenient and rapid method for the complete removal of CoA from butyryl-CoA dehydrogenase

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Why is it important?

For many years there was a puzzle over the mysterious and variable green colour of butyryl CoA dehydrogenase. Eventually was showed this is due to tightly bound CoA persulphide. In a normal catalytic assay this would be rapidly displaced, but in experiments that need a higher concentration of homogeneous enzyme CoA and its persulphide need to be removed. This paper offers a simple and cunning way of doing this.