What is it about?
Traditional methodologies in micro- and nanofluidics measure biological mechanisms as an average of a population of molecules as only their combined effect can be detected. Fluorescence fluctuation spectroscopy/imaging/nanoscopy are used as alternative experimental approaches in ultrasensitive analytics at the single-molecule level. However, what is the measurement time in which one is able to study just one single molecule in solution without immobilizing it? Existing theories are inadequate since they do not predict the meaningful time as a function of the concentration of other molecules of the same kind in bulk solution.
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Why is it important?
Experimental hypotheses differ according to which single-molecule detection methods are thought to have greater validity. As will be seen in this article, it is worthwhile to obtain a correct form of the meaningful-time relationship through theoretical means. The new ideas are comprehensively presented, and this relationship is a new concept at this time. The meaningful time for studying just one molecule without immobilization specifies the time parameter in the selfsame molecule likelihood estimator. The thermodynamic Single-Molecule DEMON: How to avoid him in the measurements of dilute liquids and live cells without immobilization or flow: https://www.linkedin.com/pulse/thermodynamic-single-molecule-demon-zeno-földes-papp/?trk=public_profile_article_view
Read the Original
This page is a summary of: What it means to measure a single molecule in a solution by fluorescence fluctuation spectroscopy, Experimental and Molecular Pathology, June 2006, Elsevier, DOI: 10.1016/j.yexmp.2006.01.001.
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Original Research Article
The thermodynamic Single-Molecule DEMON: How to avoid him in the measurements of dilute liquids and live cells without immobilization or flow: "Single-molecule time resolution in dilute liquids and live cells at the molecular scale: Constraints on the measurement time" in American Journal of Translational Medicine 2021, 5 (3), 154-165.
Publication of the Medical University Graz, Austria
Medizinische Universität Graz, Ausgabe 1, 2007, p.24: 3. AUSZEICHNUNGEN, EHRUNGEN, PREISE, BESONDERE LEISTUNGEN: Der Artikel von Univ.-Prof. DDr. Zeno Földes-Papp „What it means to measure a single molecule in a solution by fluorescence fluctuation spectroscopy • Review article“, Experimental and Molecular Pathology, Volume 80, Issue 3, 1 June 2006, Pages 209-218, war drittgereiht in der Downloadstatistik aller Fachgebiete des Journal of Experimental Molecular Pathology von Juli bis September 2006.
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