What is it about?
Unlike the others, surface charge engineering by modifying crucial surface amino acid residues is an approach of structure-based rational design that may largely ensure the catalytic efficiency while improving thermostabiliy by keeping the flexibility of the catalytic core.
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Why is it important?
The optimization of charge-charge interactions has also been proven feasible for thermostability improvement. However, underdeveloped bioinformatic tools previously limited the widespread application of surface charge engineering. We aimed to obtain optimized mutants of BisGlu16B with improved thermostability and retained enzymatic activity through strengthening charge–charge interactions on the basis of ETSS calculation.
Perspectives
Molecular dynamics simulation showed that mutation sites A47, A213, and A253 increased the protein rigidity by lowering the overall root mean square deviation (RMSD). This study may bring optimization of technology and improvement of producing efficiency to the present brewing industry.
Dr Richard Ansah Herman
Jiangsu University of Science and Technology
Read the Original
This page is a summary of: More efficient barley malting under catalyst: Thermostability improvement of a β-1,3-1,4-glucanase through surface charge engineering with higher activity, Enzyme and Microbial Technology, January 2023, Elsevier,
DOI: 10.1016/j.enzmictec.2022.110151.
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