What is it about?

Using a fluorescence assay, a fast, diffusion-controlled association event and a slower binding event were observed for a large pyrrole-imidazole polyamide associating with double-stranded DNA. The second event is 350-1500 fold slower than the first. Both association phases are seen in a 1:1 complex. Dissociation rates are quite slow, at 10^-3 s^-1.

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Why is it important?

While the characterization of PA-DNA behavior has focused principally on hairpin PAs 6–8 rings in size, there is increasing evidence that their behavior does not necessarily reflect the complexities that are emerging from studies of larger hairpin PAs, particularly concerning sequence mismatch tolerance and observed but unaddressed high PA-target site binding stoichiometries.


Using a high time-resolution method has provided more details on polyamide-DNA association.

Professor James Keane Bashkin
University of Missouri-St. Louis

Read the Original

This page is a summary of: DNA binding site kinetics of a large antiviral polyamide, Biochimie, June 2021, Elsevier,
DOI: 10.1016/j.biochi.2021.03.015.
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