HPLC-DAD-MS-SPE-UV-NMR for on-line identification of orchid metabolites

What is it about?

In our continuous efforts to study orchids, we report the identification of nine phenanthrene derivatives in a newly explored species, Aerides rosea. With the development of state-of-the art hyphenated techniques, we identified these compounds on-line by HPLC-ESI-MS/MS and HPLC-DAD-MS-SPE-NMR. All the spectral data were obtained only from microgram quantities of each metabolite. Two new phenanthrene derivatives, namely aeridin and aerosanthrene, were identified.

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Why is it important?

Orchids are precious and expensive raw material. Mainly used for ornamental uses, their chemistry remains still unexplored in details. The state-of-the art hyphenated techniques, such as HPLC-DAD-MS-SPE-NMR, allow the identification of natural products without the necessity of kilograms of plants and preserves thus the biodiversity.

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