Human feeder cells improve culture conditions for human pluripotent stem cells.

What is it about?

We discovered that human fibroblast feeder cells could secrete FGF2 and therefore maintained pluripotency of human pluripotent stem cell cultures even in the absence of supplemental FGF2. These feeders could support the pluripotency, karyotypes and proliferation of stem cells with or without FGF2 in prolonged cultures as efficiently as that on mouse-derived feeder cells.

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Why is it important?

The human feeder cells developed in this work eliminate the use of mouse feeder cells, thus making the human pluripotent stem cell cultures xeon-free, a quality sought for future clinical therapeutics using pluripotent stem cels.