What is it about?
It is a procedure for using phase contrast microscopy to obtain three-dimensional (3-D) images for the study of living cells by photographing a retinal stem living cells in a culture dish. It also include a procedure to increase the quality of scanning electron micrographs and laser confocal images. In the case of scanning electron microscopy and laser confocal images, a Gaussian procedure is applied to the original images.
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Why is it important?
The described procedure is fast, precise, simple, and offers a strategic tool for obtaining 3-D reconstructions of retinal stem cells and axons suitable for orientation and polarity of a specimen. It also enables video reconstructions. It provides the same powerful information as optical tomography, which most confocal microscopes cannot do on sterile living cells.
Perspectives
We have found a easy-cheaper way to obtain quality images from retinal spheroids and stimulated explants with vigorous outgrowth in comparison with other possible strategies. It enhance focus for living cell assays, depth, brightness, and sense of volumen. The described procedure is simple and offers a strategic tool for video reconstructions. It provides information as optical tomography on sterile living cells.
Professor Nèstor Gabriel CARRI
CONICET
Read the Original
This page is a summary of: Anaglyph of Retinal Stem Cells And Developing Axons: Selective Volume Enhancement In Microscopy Images, The Anatomical Record, February 2014, Wiley,
DOI: 10.1002/ar.22889.
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