Javier Casado
The Stem Cell Therapy Unit at CCMIJU is a research group focused in several projects based on stem cell therapies for the treatment of cardiovascular and inflammatory-related diseases
The preclinical research is a building block for increasing the understanding of stem cells biology and plays a critical role to predict the efficacy of adult stem cells in clinical trials
Preclinical trials in large animal models is a significant step for future treatments in humans, being a necessary step to evaluate the safety, feasibility, dosage, engraftment and toxicity of adoptively transferred cells. Our research group is currently focused on the applicability and therapeutic potential of microvesicles released by stem cells in the treatment of inflammatory-related diseases. Our main efforts are directed against myocardial infarction and adverse inflammatory reactions.
Industry briefing
Our research group collaborate with public institutions and private companies focused in the development of new molecules, cells or devices in the field of regenerative medicine.
Press briefing
Our research group is currently focused in the preclinical evaluation of adult stem cells. Our institution is especiallized in the evaluation of these therapies in clinically relevant animal models. We consider that large animal models are necessary to achieve a succesful clinical use of these therapies.
Slides from 25th Congress of the Spanish Society of Surgical Research, Barcelona.
ACC.20 Together With World Congress of Cardiology
Allogeneic cardiac-derived progenitor cells without immunosuppression could provide an effective ancillary therapy to improve cardiac function in reperfused MI
This study aimed to identify which are the very early immune-related biomarkers that may be used as predictors of myocardial infarction severity.
This review aims to summarize in vitro and in vivo studies where differentiated and stem cells have been combined with surgical meshes.
EV-endMSCs increase the quality of the embryos, presumably by modulating the expression of antioxidant enzymes and promoting pluripotent activity.
We describe a well-characterized ischemia-reperfusion animal model that may serve to evaluate therapeutic agents under reproducible and controlled conditions.
We describe a pre-clinical animal model for studying the pathophysiology of ischemia-reperfusion injury in reconstructive microsurgery
Hematological, biochemical and immunological parameters and Magnetic resonance derived cardiac function measurements obtained from a myocardial infarction model
Extracellular vesicles derived from endometrial mesenchymal stem cells exert an antioxidant effect and can be used as IVF coadjutants
This paper aimed to evaluate the regulatory effects of extracellular vesicles derived from endMSCs (EV‐endMSCs) in the setting of T cell activation.
Subcutaneous administration of adipose stem cells embedded on a collagen type I scaffold reduces tissue damage after ischemia-reperfusion injury in free flaps
Extracellular vesicles derived from endMSCs may be used as a co-adjuvant to improve in vitro fertilization outcomes and embryo quality.
The aim of this preclinical study was to evaluate the therapeutic effect of MSCs and their exosomes combined with FG for the treatment of incisional hernia.
Comparative the safety and effectiveness of an intracoronary (IC) infusion of microencapsulated IGF-1 and HGF in an infarcted swine model.
The immune monitoring of synovial fluids in this model allowed us the identification of IL-12p40 and T cell subsets as immune biomarkers.
Our results demonstrated a decrease of synovial lymphocytes together with a downregulation of TNF-α transcripts in those exosome-treated joints.
Here we provide a detailed analysis of bone-marrow derived MSCs and leukocyte subsets from Large White pigs and Göttingen Minipigs.
The effect of both age and cancer may act synergistically to diminish NK cell-mediated tumour immunosurveillance.
The objective of the study was to assess Roux-en-Y independent-to-weight metabolic effects on an obese Gottingen minipig.
Here we hypothe-size that Mesenchymal Stem Cells (MSCs) could be a useful tool tofavor the biocompatibility of material.
Exosomes are small membranous vesicles secreted to the extra-cellular space by most cell types through an exocytic process.
Concentrator filters forthe isolation of MSCs-derived exosomes could be an alternativeto conventional protocols using ultracentrifugation
The intrapericardial delivery has been defined as an efficient method for pharmacological agent delivery.
The combined use of surgical meshes and mesenchymal stem cells (MSCs) could be a useful tool to reduce the inflammatory reaction secondary to mesh implantation
We have evaluated the potential of genetically modified MSCs from mice bone marrow, D1-MSCs, immobilized in alginate microcapsules with different RGD (Arg-Gly-A
NK cell-based immunotherapy has emerged as a possibility for the treatment of acute myeloid leukaemia patients
The intracoronary injection of 25x10(6) allogeneic cardiac stem cells is generally safe, both early and 7 days after experimental infarction.
We demonstrate that pericardial fluid is a suitable vehicle for MSCs and intrapericardial route provide an optimal retention and implantation of MSCs.
We aimed to assess the preventive effect of an absorbable hemostatic collagen sponge on the reduction of postsurgical gynecological adhesions in a porcine model
Allogeneic cells may represent an answer, providing an off-the-shelf product that could be used in the acute stage, before the myocardial damage is irrevocable.
Released exosomes from MSCs may have an immunomodulatory role on the differentiation, activation and function of different lymphocyte subsets.
SAFETY AND EFFICACY ASSESSMENT OF INTRACORONARY DELIVERY OF PORCINE CARDIAC STEM CELLS IN A SWINE MODEL OF ACUTE MYOCARDIAL INFARCTION
Safety and efficacy assessment of early delivery and the possible influence of the timing of allogeneic pCSCs delivery after AMI on cardiac function
The intrapericardial delivery has been defined as an efficient method for pharmacological agent delivery
To benefit the overall wound healing process, we aimed to analyze the usage of pretreated sutures for improving the implantation of MSCs in the tissues.
Proinflammatory cytokines are increased in acute myeloid leukemia patients. AML patients’ survival inversely correlates with IL-6 levels.
The MSCs have strong immunomodulatory properties onNK cells through cell-to-cell interactions being mainly me-diated by the release of several soluble factors
Genetic study and differentiation behavior of the most commonly used stem cell lines for clinical practices.
hASCs and hBM-MSCs can modulate NK cells through the action of soluble factors such as indoleamine 2,3-dioxygenase.
We show reduced expression of NKp46, NKp30, DNAM-1, CD244 and CD94/NKG2C activating receptors on NK cells from acute myeloid leukaemia patients.
Expression of CD112 and CD155 on leukemic blasts induces a decreased expression of the activating receptor DNAM‐1 on NK cells from a AML patients
NK cell subsets are differentially affected by aging. Whereas CD56bright cells are decreased in healthy elderly individuals, the CD56dim subset is expanded
Overview of recent advances in the study of melanoma cells’ susceptibility to NK cell-mediated lysis
The interactions between NK cells and purified prostasomes resulted in a decrease of CD244 expression.
Expression of adhesion molecules and ligands for activating and costimulatory receptors involved in cell-mediated cytotoxicity in a human melanoma cell lines
In this study, we analyse the effect of ageing on the frequency of total and CMV-specific CD8 T cell subsets defined by the expression of CCR7 and CD45RA.
Correlation of effector function with phenotype and cell division after in vitro differentiation of naive MART-1-specific CD8+ T cells
Expression of the cancer/germ‐line antigen NY‐ESO‐1 by tumors elicits spontaneous humoral and cellular immune responses in some cancer patients.
Analysis of HNK-1 expression of melanoma cell lines, the involvement of this epitope in melanoma cell adhesion, as well as its migratory and invasive behavior
An increase of NKR CD8 T cells has been found in melanoma patients, correlating with the expansion of differentiated effector CD8 CD28null CD27null T cells
CD8 T cells expressing NK associated receptors are increased in melanoma patients and display an effector phenotype
In this paper we review how altered expression of molecules required for T-cell costimulation could result in impaired lysis of melanoma
We have studied the effect of aging on NKT cells from healthy elderly individuals.
Experimental study analysing the expression of NK-associated receptors on peripheral blood T lymphocytes from HIV-infected individuals
We describe recent data about the effects of ageing on NK cells.
Expression of NK Associated Receptors on T-Cells Is Associated with the Expansion of the Effector Memory Subset
This study demonstrates thatour in vitro expansion protocol provided a high yield of expansion being useful for subsequent clinical trials.
Implantation failure is the most common cause of recurrent pregnancy loss in humans who resort to assisted reproduction technologies
IFNγ-PRECONDITIONING TREATMENT INCREASES IMMUNE SYSTEM-RELATED PROTEINS IN EXTRACELLULAR VESICLES DERIVED FROM HUMAN ENDOMETRIAL MESENCHYMAL STROMAL CELLS
Extracellular vesicles derived from human endometrial mesenchymal stem cells improves IVF outcome in an aged murine model.
We provide evidences for an important role of immunophilins in platelet secretion and aggregation.
Phenotype, differentiation behaviour and genen expression of porcine MSCs isolated from different sources: a step forward for translational preclinical research
Absorbable surgical sutures coated with mesenchymal stem cells improve wound healing through the release of different collagen types
The usage of pre-treated sutures provided an enhanced cell adhesion and improved resitance to suture passage ensuring the presence of MSCs in the sutured tissue
This work raises the possibility of combining genetically modified ASCs that overexpress growth factors IGF1 and HGF in the same treatment in a model of AMI.
WOUND HEALING CAN BE ENHANCED BY MESENCHYMAL STEM CELL-COATED SUTURES: INVOLVEMENT OF COLLAGEN RELEASE
Histological assessment of the chondral lesion in porcine model
Exosomes derived from endometrial human mesenchymal stem cells improve the quality of in vivo derived mice embryos
Subcutaneous implantation of MSCs-Coated meshes for reducing Post-Surgical inflamamation in a murine model.
The BSA immunization induced a humoral and cellularresponse with changes on peripheral blood and synovial lym-phocytes inducing an increase of CD4+ T cell.
The aim of this work was to compare -in terms of efficiency, size and purity- different isolation protocols for human MSCs-derived exosomes.
TGF-β induce a decrease of NKp30 and not affect the expression of NKp46 and NKG2D.
Treatment of cells with H2O2 or thapsigargin resulted in activation of the proapoptotic protein Bid.
Our results support that a defective differentiation from immature CD56 negative NK cells to mature CD56dim NK cells occurs in HIV-1 infection
Analysis of expression of CD28 co-stimulatory molecules andNK-Rs in several situations that involve chronic activation of the immune system
Experimental data demonstrated that the regenerative potential and immunomodulatory capacity of cardiosphere-derived cells is mediated by paracrine mechanisms
