All Stories

  1. Engineering High-Performance Inhalable Monoclonal Antibody Powders via Spray Freeze Drying

    Article • European Journal of Pharmaceutics and Biopharmaceutics, October 2026, Elsevier

    Roberto Pisano

  2. Towards a Scalable Production of Staphylococcus epidermidis Extracellular Vesicles: Integration of Stirred-Tank Bioreactor and Ultrafiltration-Based Recovery

    Article • International Journal of Molecular Sciences, August 2026, MDPI AG

    Roberto Pisano

  3. Stabilizing Probiotics by Drying: A Review on Processes, Protective Strategies, and Viability Assessment

    Article • Processes, July 2026, MDPI AG

    Roberto Pisano

  4. Modular Design of Vacuum Systems for Lyophilization

    Article • Industrial & Engineering Chemistry Research, March 2026, American Chemical Society (ACS)

    Roberto Pisano

  5. Analysis of the Shear Stresses in a Filling Line of Parenteral Products: The Role of Peristaltic Pumping

    Article • Processes, February 2026, MDPI AG

    Roberto Pisano

  6. Molecular Drivers of Self-Assembly in RNA-Loaded lipid nanoparticles Revealed by Coarse-Grained simulations

    Article • International Journal of Pharmaceutics, January 2026, Elsevier

    Roberto Pisano

  7. Continuous lyophilization of suspended vials with per-vial inline analytics

    Article • Journal of Pharmaceutical Sciences, January 2026, Elsevier

    Roberto Pisano

  8. Controlling Mannitol Polymorphism for Enhanced Dispersibility in Spray Freeze-Dried Inhalable Microparticles

    Article • Processes, December 2025, MDPI AG

    Roberto Pisano

  9. Vacuum compatible spring wire system for mass measurement of vials during lyophilization

    Article • Precision Engineering, October 2025, Elsevier

    Roberto Pisano

  10. Mathematical modelling of thermal interactions during freezing: Effects on product morphology and drying behaviour

    Article • European Journal of Pharmaceutical Sciences, July 2025, Elsevier

    Roberto Pisano

  11. Turning Down the Inhibition Effect of Silica Gels in Protein Crystallization

    Article • ACS Applied Materials & Interfaces, June 2025, American Chemical Society (ACS)

    Roberto Pisano

  12. The impact of process parameters on the lyophilized porous micro-structure: A case study of dextran

    Article • Journal of Pharmaceutical Sciences, February 2025, Elsevier

    Roberto Pisano

  13. Enhancing Mass Transfer Coefficient Prediction from Field Emission Scanning Electron Microscope Images Through Convolutional Neural Networks and Data Augmentation Techniques

    Article • Processes, January 2025, MDPI AG

    Roberto Pisano

  14. The impact of the stopper position and geometry on the freeze-drying cycle of pharmaceutical products

    Article • Drying Technology, September 2024, Taylor & Francis

    Roberto Pisano

  15. Analysis of the shear stresses in a filling line of parenteral products: The role of sterilizing filters

    Article • Separation and Purification Technology, September 2024, Elsevier

    Roberto Pisano

  16. Understanding Glucagon Aggregation: In Silico Insights and Experimental Validation

    Article • Molecular Pharmaceutics, July 2024, American Chemical Society (ACS)

    Roberto Pisano, Professor Walter Cabri

  17. Insights into Thermal Interactions in Frozen Pharmaceutical Vials: Effects on Ice Nucleation Times and Inhibition

    Article • Pharmaceutical Research, May 2024, Springer Science + Business Media

    Roberto Pisano

  18. A review on micro and nanoengineering in Powder-Based pulmonary drug delivery

    Article • International Journal of Pharmaceutics, May 2024, Elsevier

    Roberto Pisano

  19. Chondroitin sulfate and caseinophosphopeptides doped polyurethane-based highly porous 3D scaffolds for tendon-to-bone regeneration

    Article • International Journal of Pharmaceutics, March 2024, Elsevier

    Roberto Pisano

  20. Shear stress as a driver of degradation for protein-based therapeutics: More accomplice than culprit

    Article • International Journal of Pharmaceutics, December 2023, Elsevier

    Roberto Pisano

  21. Advances in Ascorbic Acid (Vitamin C) Manufacturing: Green Extraction Techniques from Natural Sources

    Article • Processes, November 2023, MDPI AG

    Roberto Pisano

  22. The Freeze-Drying of Pharmaceuticals in Vials Nested in a Rack System—Part II: Primary Drying Behaviour

    Article • Pharmaceutics, November 2023, MDPI AG

    Roberto Pisano

  23. Tailoring Dry Microparticles for Pulmonary Drug Delivery: Ultrasonic Spray Freeze-Drying with Mannitol and Salbutamol Sulphate

    Article • Processes, October 2023, MDPI AG

    Roberto Pisano

  24. Comparative Studies of Different Preservation Methods and Relative Freeze-Drying Formulations for Extracellular Vesicle Pharmaceutical Applications

    Article • ACS Biomaterials Science & Engineering, September 2023, American Chemical Society (ACS)

    Roberto Pisano

  25. New Trends in Freeze-Drying of Pharmaceutical Products

    Article • Pharmaceutics, July 2023, MDPI AG

    Roberto Pisano

  26. Thermodynamic Modeling and Experimental Data Reveal That Sugars Stabilize Proteins According to an Excluded Volume Mechanism

    Article • Journal of the American Chemical Society, July 2023, American Chemical Society (ACS)

    Roberto Pisano

  27. Mechanistic Investigation of tert-Butanol’s Impact on Biopharmaceutical Formulations: When Experiments Meet Molecular Dynamics

    Article • Molecular Pharmaceutics, July 2023, American Chemical Society (ACS)

    Roberto Pisano

  28. A diffused-interface model for the lyophilization of a packed bed of spray-frozen particles

    Article • Chemical Engineering Science, July 2023, Elsevier

    Roberto Pisano

  29. Analysis of the Shear Stresses in a Filling Line of Parenteral Products: The Role of Fittings

    Article • Processes, June 2023, MDPI AG

    Roberto Pisano

  30. Self-Assembled Monolayers As a Tool to Investigate the Effect of Surface Chemistry on Protein Nucleation

    Article • Crystal Growth & Design, March 2023, American Chemical Society (ACS)

    Roberto Pisano, Dr. Jose A. Gavira

  31. Analysis of the Shear Stresses in a Filling Line of Parenteral Products: The Role of Tubing

    Article • Processes, March 2023, MDPI AG

    Roberto Pisano

  32. On the Use of Temperature Measurements as a Process Analytical Technology (PAT) for the Monitoring of a Pharmaceutical Freeze-Drying Process

    Article • Pharmaceutics, March 2023, MDPI AG

    Roberto Pisano

  33. Freeze-Drying of Pharmaceuticals in Vials Nested in a Rack System—Part I: Freezing Behaviour

    Article • Pharmaceutics, February 2023, MDPI AG

    Roberto Pisano

  34. Modernizing Manufacturing of Parenteral Products

    Article • December 2022, Taylor & Francis

    Roberto Pisano

  35. Cytotoxicity and Thermal Characterization Assessment of Excipients for the Development of Innovative Lyophilized Formulations for Oncological Applications

    Article • Processes, December 2022, MDPI AG

    Roberto Pisano

  36. A New Transfer Free Energy Based Implicit Solvation Model for the Description of Disordered and Folded Proteins

    Article • The Journal of Physical Chemistry B, August 2022, American Chemical Society (ACS)

    Roberto Pisano

  37. An Experimental and Modeling Combined Approach in Preparative Hydrophobic Interaction Chromatography

    Article • Processes, May 2022, MDPI AG

    Roberto Pisano

  38. Combining Mathematical Modeling and Thermal Infrared Data in the Freezing of Pharmaceutical Liquid Formulations

    Article • Industrial & Engineering Chemistry Research, March 2022, American Chemical Society (ACS)

    Roberto Pisano

  39. Ultrasonic spray freeze-drying of sucrose and mannitol-based formulations: Impact of the atomization conditions on the particle morphology and drying performance

    Article • Drying Technology, January 2022, Taylor & Francis

    Roberto Pisano

  40. Lipid-Based Nanovesicular Drug Delivery Systems

    Article • Nanomaterials, December 2021, MDPI AG

    Roberto Pisano

  41. Use of Microfluidic Capillary Electrophoresis for the Determination of Multi-Component Protein Adsorption Isotherms: Application to High-Throughput Analysis for Hydrophobic Interaction Chromatography

    Article • Pharmaceutics, December 2021, MDPI AG

    Roberto Pisano

  42. Surface Treatment of Glass Vials for Lyophilization: Implications for Vacuum-Induced Surface Freezing

    Article • Pharmaceutics, October 2021, MDPI AG

    Roberto Pisano

  43. Investigation of the Freezing Phenomenon in Vials Using an Infrared Camera

    Article • Pharmaceutics, October 2021, MDPI AG

    Roberto Pisano

  44. Alginate Nanohydrogels as a Biocompatible Platform for the Controlled Release of a Hydrophilic Herbicide

    Article • Processes, September 2021, MDPI AG

    Roberto Pisano

  45. Effect of Polymerization Time on the Binding Properties of Ciprofloxacin-Imprinted nanoMIPs Prepared by Solid-Phase Synthesis

    Article • Polymers, August 2021, MDPI AG

    Roberto Pisano

  46. Force Field Parameterization for the Description of the Interactions between Hydroxypropyl-β-Cyclodextrin and Proteins

    Article • The Journal of Physical Chemistry B, July 2021, American Chemical Society (ACS)

    Roberto Pisano

  47. In-Line Monitoring of the Freeze-Drying Process by Means of Heat Flux Sensors

    Article • Industrial & Engineering Chemistry Research, June 2021, American Chemical Society (ACS)

    Roberto Pisano

  48. The Role of Cyclodextrins against Interface-Induced Denaturation in Pharmaceutical Formulations: A Molecular Dynamics Approach

    Article • Molecular Pharmaceutics, May 2021, American Chemical Society (ACS)

    Roberto Pisano

  49. Tuning Transport Phenomena in Agarose Gels for the Control of Protein Nucleation Density and Crystal Form

    Article • Crystals, April 2021, MDPI AG

    Roberto Pisano, Dr. Jose A. Gavira

  50. Role of Self-Assembled Surface Functionalization on Nucleation Kinetics and Oriented Crystallization of a Small-Molecule Drug: Batch and Thin-Film Growth of Aspirin as a Case Study

    Article • ACS Applied Materials & Interfaces, March 2021, American Chemical Society (ACS)

    Roberto Pisano

  51. Enhancing the preservation of liposomes: The role of cryoprotectants, lipid formulations and freezing approaches

    Article • Cryobiology, January 2021, Elsevier

    Roberto Pisano

  52. Economic Analysis of a Freeze-Drying Cycle

    Article • Processes, November 2020, MDPI AG

    Roberto Pisano

  53. ADD Force Field for Sugars and Polyols: Predicting the Additivity of Protein–Osmolyte Interaction

    Article • The Journal of Physical Chemistry B, August 2020, American Chemical Society (ACS)

    Roberto Pisano

  54. Agarose Gel as a Medium for Growing and Tailoring Protein Crystals

    Article • Crystal Growth & Design, July 2020, American Chemical Society (ACS)

    Roberto Pisano, Dr. Jose A. Gavira

  55. General and adaptive synthesis protocol for high-quality organosilane self-assembled monolayers as tunable surface chemistry platforms for biochemical applications

    Article • Biointerphases, July 2020, American Vacuum Society

    Roberto Pisano

  56. Spray Freeze-Drying as a Solution to Continuous Manufacturing of Pharmaceutical Products in Bulk

    Article • Processes, June 2020, MDPI AG

    Roberto Pisano

  57. Automatic control of a freeze-drying process: Detection of the end point of primary drying

    Article • Drying Technology, June 2020, Taylor & Francis

    Roberto Pisano

  58. A model-based approach for the rational design of the freeze-thawing of a protein-based formulation

    Article • Pharmaceutical Development and Technology, May 2020, Taylor & Francis

    Roberto Pisano

  59. Heightened Cold-Denaturation of Proteins at the Ice–Water Interface

    Article • Journal of the American Chemical Society, March 2020, American Chemical Society (ACS)

    Roberto Pisano

  60. Impact of Controlled Vacuum Induced Surface Freezing on the Freeze Drying of Human Plasma

    Article • International Journal of Pharmaceutics, March 2020, Elsevier

    Roberto Pisano

  61. Synthesis of high payload nanohydrogels for the ecapsulation of hydrophilic molecules via inverse miniemulsion polymerization: caffeine as a case study

    Article • Drug Development and Industrial Pharmacy, October 2019, Taylor & Francis

    Roberto Pisano

  62. From Batch to Continuous: Freeze-Drying of Suspended Vials for Pharmaceuticals in Unit-Doses

    Article • Industrial & Engineering Chemistry Research, January 2019, American Chemical Society (ACS)

    Roberto Pisano

  63. Tuning, measurement and prediction of the impact of freezing on product morphology: A step toward improved design of freeze-drying cycles

    Article • Drying Technology, January 2019, Taylor & Francis

    Roberto Pisano

  64. Supporting data and methods for the multi-scale modelling of freeze-drying of microparticles in packed-beds

    Article • Data in Brief, December 2018, Elsevier

    Roberto Pisano

  65. A multi-scale computational framework for modeling the freeze-drying of microparticles in packed-beds

    Article • Powder Technology, November 2018, Elsevier

    Roberto Pisano

  66. Process intensification and process control in freeze-drying

    Article • September 2018, Universitat Politecnica de Valencia

    Roberto Pisano

  67. Effect of Surfactants on Surface-Induced Denaturation of Proteins: Evidence of an Orientation-Dependent Mechanism

    Article • The Journal of Physical Chemistry B, September 2018, American Chemical Society (ACS)

    Roberto Pisano

  68. Looking inside the ‘black box’: Freezing engineering to ensure the quality of freeze-dried biopharmaceuticals

    Article • European Journal of Pharmaceutics and Biopharmaceutics, August 2018, Elsevier

    Roberto Pisano

  69. Surface-induced crystallization of pharmaceuticals and biopharmaceuticals: A review

    Article • International Journal of Pharmaceutics, August 2018, Elsevier

    Roberto Pisano

  70. Surfactants as stabilizers for biopharmaceuticals: An insight into the molecular mechanisms for inhibition of protein aggregation

    Article • European Journal of Pharmaceutics and Biopharmaceutics, July 2018, Elsevier

    Roberto Pisano

  71. Vacuum Induced Surface Freezing as an effective method for improved inter- and intra-vial product homogeneity

    Article • European Journal of Pharmaceutics and Biopharmaceutics, July 2018, Elsevier

    Roberto Pisano

  72. The Preservation of Lyophilized Human Growth Hormone Activity: how Do Buffers and Sugars Interact?

    Article • Pharmaceutical Research, April 2018, Springer Science + Business Media

    Roberto Pisano

  73. Process analytical technology for monitoring pharmaceuticals freeze-drying – A comprehensive review

    Article • Drying Technology, March 2018, Taylor & Francis

    Roberto Pisano

  74. Measuring and predicting pore size distribution of freeze-dried solutions

    Article • Drying Technology, February 2018, Taylor & Francis

    Roberto Pisano

  75. Water entrapment and structure ordering as protection mechanisms for protein structural preservation

    Article • The Journal of Chemical Physics, February 2018, American Institute of Physics

    Roberto Pisano

  76. Clarifying the role of cryo- and lyo-protectants in the biopreservation of proteins

    Article • Physical Chemistry Chemical Physics, January 2018, Royal Society of Chemistry

    Roberto Pisano

  77. Inclusion complexes dispersed in polystyrene-based labels for fruit ripening on demand

    Article • International Journal of Food Science & Technology, September 2017, Wiley

    Roberto Pisano

  78. Stability of Proteins in Carbohydrates and Other Additives during Freezing: The Human Growth Hormone as a Case Study

    Article • The Journal of Physical Chemistry B, September 2017, American Chemical Society (ACS)

    Roberto Pisano

  79. Prediction of product morphology of lyophilized drugs in the case of Vacuum Induced Surface Freezing

    Article • Chemical Engineering Research and Design, September 2017, Elsevier

    Roberto Pisano

  80. Prediction of Ice Crystal Size Distribution after Freezing of Pharmaceutical Solutions

    Article • Crystal Growth & Design, August 2017, American Chemical Society (ACS)

    Roberto Pisano

  81. Impact of vacuum-induced surface freezing on inter- and intra-vial heterogeneity

    Article • European Journal of Pharmaceutics and Biopharmaceutics, June 2016, Elsevier

    Roberto Pisano

  82. Non-Invasive Temperature Monitoring in Freeze Drying: Control of Freezing as a Case Study

    Article • Drying Technology, June 2015, Taylor & Francis

    Roberto Pisano

  83. Computer-Aided Framework for the Design of Freeze-Drying Cycles: Optimization of the Operating Conditions of the Primary Drying Stage

    Article • Processes, May 2015, MDPI AG

    Roberto Pisano

  84. Wavelet image decomposition for characterization of freeze-dried pharmaceutical product structures

    Article • May 2015, Institute of Electrical & Electronics Engineers (IEEE)

    Roberto Pisano

  85. Effect of Electron Beam Irradiation on Remaining Activity of Lyophilized Acid Phosphatase with Water-Binding and Non-Water–Binding Additives

    Article • Drying Technology, December 2014, Taylor & Francis

    Roberto Pisano

  86. Vacuum-Induced Nucleation as a Method for Freeze-Drying Cycle Optimization

    Article • Industrial & Engineering Chemistry Research, November 2014, American Chemical Society (ACS)

    Roberto Pisano

  87. Tailoring of Bone Scaffold Properties Using Silicate/Phosphate Glass Mixtures

    Article • Key Engineering Materials, November 2014, Trans Tech Publications

    Professor Julian R Jones, Roberto Pisano, PhD Giorgia Novajra

  88. A New Method Based on the Regression of Step Response Data for Monitoring a Freeze-Drying Cycle

    Article • Journal of Pharmaceutical Sciences, June 2014, Elsevier

    Roberto Pisano

  89. Characterization of freeze-dried pharmaceutical product structures by an FFT-imaging approach

    Article • June 2014, Institute of Electrical & Electronics Engineers (IEEE)

    Roberto Pisano

  90. Real-time temperature monitoring in pharmaceutical freeze-drying

    Article • June 2014, Institute of Electrical & Electronics Engineers (IEEE)

    Roberto Pisano

  91. Sputtered thermocouple array for vial temperature mapping

    Article • May 2014, Institute of Electrical & Electronics Engineers (IEEE)

    Roberto Pisano

  92. Applying quality-by-design to develop a coffee freeze-drying process

    Article • Journal of Food Engineering, February 2014, Elsevier

    Roberto Pisano

  93. Intensification of Freeze‐Drying for the Pharmaceutical and Food Industries

    Article • January 2014, Wiley

    Roberto Pisano

  94. Freeze-drying of enzymes in case of water-binding and non-water-binding substrates

    Article • European Journal of Pharmaceutics and Biopharmaceutics, November 2013, Elsevier

    Roberto Pisano

  95. Quality by Design: Scale-Up of Freeze-Drying Cycles in Pharmaceutical Industry

    Article • AAPS PharmSciTech, July 2013, American Association of Pharmaceutical Scientists (AAPS)

    Roberto Pisano

  96. In-Line and Off-Line Optimization of Freeze-Drying Cycles for Pharmaceutical Products

    Article • Drying Technology, June 2013, Taylor & Francis

    Roberto Pisano

  97. Quality by design: optimization of a freeze-drying cycle via design space in case of heterogeneous drying behavior and influence of the freezing protocol

    Article • Pharmaceutical Development and Technology, October 2012, Taylor & Francis

    Roberto Pisano

  98. Model-Based Framework for the Analysis of Failure Consequences in a Freeze-Drying Process

    Article • Industrial & Engineering Chemistry Research, September 2012, American Chemical Society (ACS)

    Roberto Pisano

  99. Quality by Design in the Secondary Drying Step of a Freeze-Drying Process

    Article • Drying Technology, September 2012, Taylor & Francis

    Roberto Pisano

  100. A Model-Based Framework to Optimize Pharmaceuticals Freeze Drying

    Article • Drying Technology, July 2012, Taylor & Francis

    Roberto Pisano

  101. Innovation in Monitoring Food Freeze Drying

    Article • Drying Technology, December 2011, Taylor & Francis

    Roberto Pisano

  102. Advanced approach to build the design space for the primary drying of a pharmaceutical freeze‐drying process

    Article • Journal of Pharmaceutical Sciences, November 2011, Elsevier

    Roberto Pisano

  103. Heat Transfer in Freeze-Drying Apparatus

    Article • September 2011, IntechOpen

    Roberto Pisano

  104. Freeze-Drying Cycle Optimization Using Model Predictive Control Techniques

    Article • Industrial & Engineering Chemistry Research, May 2011, American Chemical Society (ACS)

    Roberto Pisano

  105. Monitoring of the Secondary Drying in Freeze-Drying of Pharmaceuticals

    Article • Journal of Pharmaceutical Sciences, February 2011, Elsevier

    Roberto Pisano

  106. On the Methods Based on the Pressure Rise Test for Monitoring a Freeze-Drying Process

    Article • Drying Technology, December 2010, Taylor & Francis

    Roberto Pisano

  107. In-Line Optimization and Control of an Industrial Freeze-Drying Process for Pharmaceuticals

    Article • Journal of Pharmaceutical Sciences, November 2010, Elsevier

    Roberto Pisano

  108. Model-Based Monitoring and Control of Industrial Freeze-Drying Processes: Effect of Batch Nonuniformity

    Article • Drying Technology, May 2010, Taylor & Francis

    Roberto Pisano

  109. In-line control of the lyophilization process. A gentle PAT approach using software sensors

    Article • International Journal of Refrigeration, August 2009, Elsevier

    Roberto Pisano

  110. Freeze Drying of Pharmaceutical Excipients Close to Collapse Temperature: Influence of the Process Conditions on Process Time and Product Quality

    Article • Drying Technology, June 2009, Taylor & Francis

    Roberto Pisano

  111. On the Design of an In-Line Control System for a Vial Freeze-Drying Process: The Role of Chamber Pressure

    Article • Chemical Product and Process Modeling, May 2009, De Gruyter

    Roberto Pisano

  112. Monitoring of the primary drying of a lyophilization process in vials

    Article • Chemical Engineering and Processing - Process Intensification, January 2009, Elsevier

    Roberto Pisano

  113. Observer design for the Selective Catalytic Reduction of NO in a loop reactor

    Article • Chemical Engineering Journal, April 2007, Elsevier

    Roberto Pisano