All Stories

  1. Mapping the effects of specific radiation damage and solvent radiolysis in buffers and crystals with online UV–Vis absorption spectroscopy
  2. Coupled on-line in crystallo UV–Vis absorption spectroscopy and X-ray crystallography to compare specific radiation damage in metal-containing proteins at room versus cryogenic temperature
  3. Time-Resolved Crystallography: From Femtoseconds to Minutes at XFELs and Synchrotrons
  4. Slow protein dynamics probed by time-resolved oscillation crystallography at room temperature
  5. Millisecond time-resolved serial oscillation crystallography of a blue-light photoreceptor at a synchrotron
  6. Specific radiation damage is a lesser concern at room temperature
  7. Structural analysis of the bright monomeric yellow-green fluorescent protein mNeonGreen obtained by directed evolution
  8. Gas-sensitive biological crystals processed in pressurized oxygen and krypton atmospheres: deciphering gas channels in proteins using a novel `soak-and-freeze' methodology
  9. Structural Determinants of Improved Fluorescence in a Family of Bacteriophytochrome-Based Infrared Fluorescent Proteins: Insights from Continuum Electrostatic Calculations and Molecular Dynamics Simulations
  10. Serial Femtosecond Crystallography and Ultrafast Absorption Spectroscopy of the Photoswitchable Fluorescent Protein IrisFP
  11. Experimental Determination of Single- and Two-Photon Excitation Transition Moments in Representative Fluorescent Proteins
  12. Rational design of a monomeric and photostable far-red fluorescent protein for fluorescence imaging in vivo
  13. Structural basis for sensitivity of fluorescent proteins to molecular oxygen investigated by high-pressure crystallography
  14. The status of the macromolecular crystallography beamlines at the European Synchrotron Radiation Facility
  15. Bacteriorhodopsin: Would the real structural intermediates please stand up?
  16. In crystallooptical spectroscopy (icOS) as a complementary tool on the macromolecular crystallography beamlines of the ESRF
  17. Experimental Determination of Transition Dipole Moment Directions in Representative Fluorescent Proteins
  18. Direct Evidence for a Peroxide Intermediate and a Reactive Enzyme-Substrate-Dioxygen Configuration in a Cofactor-free Oxidase
  19. Direct Evidence for a Peroxide Intermediate and a Reactive Enzyme-Substrate-Dioxygen Configuration in a Cofactor-free Oxidase
  20. Nanoparticle Surface-Enhanced Raman Scattering of Bacteriorhodopsin Stabilized by Amphipol A8-35
  21. In crystallo optical spectroscopy as a complementary tool to crystallography
  22. Pressure and high-pressure macromolecular crystallography at the ESRF
  23. Structures of a human blood group glycosyltransferase in complex with a photo-activatable UDP-Gal derivative reveal two different binding conformations
  24. An improved monomeric infrared fluorescent protein for neuronal and tumour brain imaging
  25. In-house UV radiation-damage-induced phasing of selenomethionine-labeled protein structures
  26. Alteration of fluorescent protein spectroscopic properties upon cryoprotection
  27. Structural Characterization of Bacterioferritin from Blastochloris viridis
  28. Structure of a fluorescent protein fromAequorea victoriabearing the obligate-monomer mutation A206K
  29. Structure-guided evolution of cyan fluorescent proteins towards a quantum yield of 93%
  30. Laser-Triggered Single Molecular Gating Motions of the KcsA Potassium Channels Recorded in a Sub-Millisecond Time Resolution
  31. Raman-assisted crystallography of biomolecules at the synchrotron: Instrumentation, methods and applications
  32. Stabilizing role of glutamic acid 222 in the structure of Enhanced Green Fluorescent Protein
  33. Raman-Assisted Crystallography Suggests a Mechanism of X-Ray-Induced Disulfide Radical Formation and Reparation
  34. Intrinsic Dynamics in ECFP and Cerulean Control Fluorescence Quantum Yield
  35. Mammalian Expression of Infrared Fluorescent Proteins Engineered from a Bacterial Phytochrome
  36. Simultaneous Measurements of Solvent Dynamics and Functional Kinetics in a Light-Activated Enzyme
  37. Crystal structure of plant light-harvesting complex shows the active, energy-transmitting state
  38. Advances in spectroscopic methods for biological crystals. 2. Raman spectroscopy
  39. Advances in spectroscopic methods for biological crystals. 1. Fluorescence lifetime measurements
  40. Use of a `caged' analogue to study the traffic of choline within acetylcholinesterase by kinetic crystallography
  41. Spectroscopic Characterization of Bacteriorhodopsin's L-intermediate in 3D Crystals Cooled to 170 K¶
  42. X-ray radiation-induced damage in DNA monitored by online Raman
  43. Advances in kinetic protein crystallography
  44. Structure of Superoxide Reductase Bound to Ferrocyanide and Active Site Expansion upon X-Ray-Induced Photo-Reduction
  45. Temperature Derivative Fluorescence Spectroscopy as a Tool to Study Dynamical Changes in Protein Crystals
  46. Deformation of Helix C in the Low Temperature L-intermediate of Bacteriorhodopsin
  47. Structural basis for sensory rhodopsin function
  48. Bacteriorhodopsin: a high-resolution structural view of vectorial proton transport
  49. Early structural rearrangements in the photocycles of bacteriorhodopsin and sensory rhodopsin II
  50. Detection and characterization of merohedral twinning in two protein crystals: bacteriorhodopsin and p67phox
  51. Early Structural Rearrangements in the Photocycle of an Integral Membrane Sensory Receptor
  52. Structural Determinants of Spectral Tuning in Retinal ProteinsBacteriorhodopsin vs Sensory Rhodopsin II #
  53. X-ray structure of sensory rhodopsin II at 2.1-Å resolution
  54. Spectroscopic Characterization of Bacteriorhodopsin's L-intermediate in 3D Crystals Cooled to 170 K¶
  55. Detergent-free membrane protein crystallization
  56. Protein, lipid and water organization in bacteriorhodopsin crystals: a molecular view of the purple membrane at 1.9 Å resolution