Influence of Plant Growth Regulators on In vitro Morphogenesis in Plumbago Zeylanica Linn.

What is it about?

The Present investigation was aimed to recognize the most responding explants, plant growth regulators and calculate their optimal concentrations and other physical aspects unveiling in vitro morphogenesis in higher rates via culturing nodal sections and leaf discs explants. Leaf disc and nodal section explants of Plumbago zeylanica were inoculated on basal media amended with diverse concentrations and combinations of different auxins and cytokinins as alone as well as in amalgamations. For inoculated nodal section, nutrient medium MS2N.5B (MS+2. 0 mgl-1 NAA+ 0. 5mgl-1 BA) evidenced appropriate for higher degree of callus initiation (91.69%), whereas culture medium MS2B (MS+2.0 mg l-1 BA) displayed higher shoot proliferating competence (84.14%). While, nutrient medium MS2B.5N (MS+2.0 mg l-1 BA+0.5 mgl-1 NAA) formed shoot(s) in higher numbers (11.12) along with of bigger length (7.11 cm). For cultured leaf disc, inoculation medium MS2N (MS+ 2.0 mgl-1 NAA) induced callus in higher percentage (91.12%), nevertheless greater morphogenic calli formation (45.58%) and plantlets regeneration were attained on culture medium MSN.5Td (MS+1.0mgl-1 NAA + 0.5 mgl-1 TDZ). In current investigation, MS medium amended with either IBA or NAA at the concentration of 0.1 mg l-1 was proved to be optimal for inducing higher in vitro rooting response i.e., root proliferation, number(s) of roots and root length. Regenerants were established efficaciously under the field conditions after hardening with normal phenotypic characters

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Why is it important?

uring the current study, we used the novel technique for nodal segmentcultures, which may be attained from plants propagated in orchard as well as invitro regenerated plantlets. The protocol developed for production of plantlets ofPlumbago zeylanica may be employed consistently for propagation in acommercial scale and ex situ conservation of this imperative medicinal plantspecies. Leaf derived callus cultures may be exploited further for isolation ofprotoplast and genetic transformation. Root could be employed as explant sourcefor deriving cell suspension cultures for producing and enhancing importantsecondary metabolites of chitrak by elicitation and immobilization procedures (PDF) Influence of Plant Growth Regulators on In vitro Morphogenesis in Plumbago Zeylanica Linn.. Available from: https://www.researchgate.net/publication/362901795_Influence_of_Plant_Growth_Regulators_on_In_vitro_Morphogenesis_in_Plumbago_Zeylanica_Linn [accessed Oct 09 2022].