Plant Regeneration in Rauvolfia serpentina (L.) Benth via Organogenic Mode

What is it about?

An effectual protocol for rapid propagation of Rauvolfia serpentina has been standardized in present investigation. Leaf discs and nodal segments were inoculated on MS medium supplemented with diverse auxins and cytokinins in variable concentrations as individual along with in different amalgamations. to recognize the most responding explants, plant growth regulators and calculate their optimum concentrations and other culture conditions revealing in vitro morphogenesis tracked by plantlet regeneration in higher frequencies by means of nodal segments and leaf discs culture of locally adapted cultivar. For nodal segment culture, basal MS media amended with 2, 4-D/ NAA at the concentration of 2.0 mgl-1 as alone or in association with 0.5mgl-1 TDZ were evidenced meaningly better for callus initiation. However, higher shoot proliferating competence, numbers of shoots per explant with maximum length were recognized on inoculation media reinvigorated with TDZ in the range of 0.2-0.5 mgl-1 as sole as well as in association with 0.5mgl-1 NAA (more than 92% nodal segments initiated shoots, >10.5 shoots/explant and mean shoot length>3.85 cm). Nevertheless, for inoculated leaf disc, maximum callus and morphogenic callus formation along with plantlet regeneration were investigated on culture media supplemented with 2.0 mgl-1 auxins: 2,4-D and/or NAA in amalgamation with 0.5mgl-1 of a cytokinin BA and/or TDZ (more than 76% leaf discs initiated morphogenic calli with >80% regeneration frequencies). Rooting Research Aspects in Biological Science Vol. 7 Plant Regeneration in Rauvolfia serpentina (L.) Benth via Organogenic Mode 125 medium MS modified with 0.1 mgl-1 IBA demonstrated a higher in vitro rooting response (root proliferating efficiency, number of roots with higher length).

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Why is it important?

The findings of current investigation established that under circumstances ofthese experimentations, in vitro response of both explant cultures was undergenetic control. As between two assorted explants cultured on diverse nutrientmedia combinations noteworthy differences were documented for all the culturephases. In conclusion in current study, we employed the novel technique in nodalsegment and leaf disc cultures, that may be attained from plants propagated inorchard along with plantlets regenerated in vitro. The established protocol maybe used for production of plantlets of sarpgandha on a commercial scale and exsitu conservation of this important medicinal plant species. Leaf derived calluscultures can be exploited further for isolation of totipotent protoplast and genetictransformation. These formulations may also be employed for root culture forderiving cell suspension cultures for producing and enhancing many importantsecondary metabolites of sarpgandha by elicitation and immobilizationprocedures (PDF) Plant Regeneration in Rauvolfia serpentina (L.) Benth via Organogenic Mode. Available from: https://www.researchgate.net/publication/362903830_Plant_Regeneration_in_Rauvolfia_serpentina_L_Benth_via_Organogenic_Mode [accessed Oct 09 2022].