Publication
Figure 10. Projection difference maps.
( A ) Superposition of the three-dimensional MjNhaP1 outward-open structure at pH 4 without sodium (blue) and the inward-open structure at pH 8 with sodium (red). Helices are shown as cylinders as seen from the cytoplasmic side. ( B ) 6 Å projection difference map calculated between the structures shown in ( A ). Major difference peaks are observed in the 6-helix bundle, whereas difference peaks at the dimer interface are weak. ( C ) Superposition of the inward-open and outward-open MjNhaP1 structures on the projection difference map shown in ( B ). ( D ) 6 Å projection difference map between MjNhaP1 2D crystals at pH 8 with and without sodium (adapted from Figure 5 in (Paulino and Kühlbrandt, 2014) top row, 500 mM NaCl brought to the same phase origin). The projection difference map calculated from the 3D structures closely resembles the projection map that shows sodium-induced changes in Paulino and Kühlbrandt (2014). The conformational change from the inward-open to outward-open st
eLife
DOI: 10.7554/elife.03583.024