Publication
Figure 7. miR-124 acts through regulation of transformer .
( A ) Elevated expression of tra F transcript measured by quantitative real-time PCR using RNA isolated from male flies of the indicated genotypes (primer pair p3 and p4). actin 42A was used as an internal control for normalization. Data represent the average of five independent experiments ± SEM. Although tra F transcript levels are low in control males, they were detected by quantitative real-time PCR (traces are shown in Figure 6). ( B ) Percentage of males achieving copulation with CS females in 30 min. Data represent the mean of more than 20 movies per genotype ± SD. Genotypes: CS: canton S control; miR-124 refers to the trans-heterozygous mutant combination miR-124 Δ4 /miR-124 Δ177 ; 124>tra RNAi refers to the trans-heterozygous mutant combination miR-124 Δ4 /miR-124 Δ177 carrying the miR-124 -promoter Gal4 transgene and UAS-tra RNAi . Depletion of tra significantly improved performance of the miR-124 mutant males. ( C ) Courtship index comparing proportion of time CS
eLife
DOI: 10.7554/elife.00640.013