What is it about?
This preprint examines how rate of oxygen provision affects E. coli growth and types of metabolites secreted. At the same time, possible toxicity effect from polyethylene glycol antifoam on cell growth was monitored at the scale of a 1L bioreactor.
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Why is it important?
Since cell growth rate affects economics of fermentation such as bioreactor utilization rate, it is important to understand how rate of oxygen provision affects cell growth. In addition, given that higher rate of oxygen provision would result in greater foam formation that, in turn, increases the chances of contamination, effect of antifoam concentration on cell growth needs to be examined to screen for toxicity effect on cells.
Perspectives
Overall, preliminary data highlighted in this preprint would help researchers understand the range of antifoam and oxygen provision rate that would not adversely affect growth of E. coli in a 1L bioreactor with LB growth medium.
Wenfa Ng
National University of Singapore
Read the Original
This page is a summary of: Effect of different aeration and polyethylene glycol concentration on growth of Escherichia coli DH5α in a 1L bioreactor, June 2018, PeerJ,
DOI: 10.7287/peerj.preprints.27014v1.
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