Comparison of Stomaching versus Rinsing for Recovering Bacterial Communities from Rainbow Trout (Oncorhynchus mykiss) Fillets

What is it about?

The study of bacterial communities on food matrix requires an efficient recovery of the bacteria. Efficiency of recovery methods relies on technical criteria (quantitative recovery, compatibility with end-point qPCR) and cost-effectiveness. We evaluated two commonly used methods (mechanical rinsing and stomaching) for the recovery of a marker bacteria from a rainbow trout fillets matrix. Mechanical rinsing allows the best recovery of initially present bacteria and efficient qPCR. We recommend to use this method to analyse the bacterial communities from a food matrix.

Featured Image