In House Validation of a Rinse-Membrane Filtration Method for Processing Fresh Produce Samples for Downstream Cultural Detection of Salmonella, Escherichia coli O157:H7, and Listeria.

What is it about?

The purpose of this study was to perform an in house-validation of a rinse-membrane filtration method to detect Salmonella, Listeria and E. coli O157:H7, determine its sensitivity, specificity and relative accuracy parameters; and compare its performance to two other frequently used processing methods: sponge rubbing and homogenization by stomacher. For these analyses we used jalapeno pepper and cantaloupe, that have been responsible for foodborne outbreaks in various countries. _x000D_ Overall, we found that the rinse-membrane filtration method performed similar or better than the homogenization and sponge surface rubbing methods, and is a good alternative for processing large numbers of produce samples for bacterial pathogen detection._x000D_

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