Antibiogram of Multidrug-Resistant Isolates of Pseudomonas aeruginosa after Biofield Treatment

What is it about?

In recent years, prevalence of multidrug resistance (MDR) in Pseudomonas aeruginosa (P. aeruginosa) has been noticed with high morbidity and mortality. Aim of the present study was to determine the impact of Mr. Trivedi’s biofield treatment on MDR clinical lab isolates (LS) of P. aeruginosa. Five MDR clinical lab isolates (LS 22, LS 23, LS 38, LS 47, and LS 58) of P. aeruginosa were taken and divided into two groups i.e. control and biofield treated. Control and treated group were analyzed for antimicrobial susceptibility pattern, minimum inhibitory concentration (MIC), biochemical study and biotype number using MicroScan Walk-Away® system. The analysis was done on day 10 after biofield treatment as compared with control group. Antimicrobial sensitivity assay showed 60% alteration in sensitivity of tested antimicrobials in MDR isolates of P. aeruginosa after biofield treatment. MIC results showed an alteration in 42.85% tested antimicrobials out of twenty eight after biofield treatment in five isolates of MDR P. aeruginosa. Biochemical study showed a 48.48% change in tested biochemical reactions out of thirty three as compared to control. A significant change in biotype numbers was reported in three clinical lab isolates of MDR P. aeruginosa out of five, after biofield treatment as compared to respective control. On the basis of changed biotype number (7302 0052) in biofield treated LS 23, new organism was identified as Citrobacter freundii as compared to control (0206 3336). A very rare biotype number (7400 4263) was found in biofield treated LS 38, as compared to control (0206 3736). Study results suggest that biofield treatment on lab isolates of MDR P. aeruginosa has significant effect on the antimicrobial sensitivity, MIC values, biochemical reactions and biotype number. Biofield treatment might prevent the emergence of absolute resistance pattern of useful antimicrobials against MDR isolates of P. aeruginosa.

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Why is it important?

Antimicrobial agents are widely used therapeutic option against infections caused by pathogenic microbes. However, through different strategies and mechanism, these microorganisms combat the effect of antimicrobial agents, as multidrug resistant (MDR) clinical strains are the best example world-wide. Pseudomonas aeruginosa (P. aeruginosa) is a ubiquitous, gram-negative bacterium and versatile opportunistic pathogen, associated with nosocomial infections along with other serious implications with high rate of morbidity and mortality [1]. Increasing resistant towards the available antimicrobials preclude the effectiveness of any antimicrobial regimen [2,3]. Because of increasing MDR P. aeruginosa isolates in health care setting, infections are difficult to treat, causing life threating conditions [4]. P. aeruginosa is one of the major pathogen related with hospital acquired infections especially in Intensive care unit [5]. According to the report of nosocomial infection surveillance system of center for disease control and prevention, P. aeruginosa is second most common cause of nosocomial pneumonia, third most common in nosocomial urinary tract infections and eighth most common cause of nosocomial bacteraemia [6]. MDR mechanism in P. aeruginosa are due to acquisition of resistance genes (β-lactamases) or because of amino-glycoside modifying enzymes [7], or due to chromosomal genes mutation involved against antimicrobials [8]. Despite of several advances in medical sciences, new generation antimicrobials against MDR strains of P. aeruginosa associated infections are still a serious challenge [9]. Recently, an alternate approach called biofield treatment is reported with effectively inhibiting the growth of bacterial cultures [10]. Biofield is the name given to the electromagnetic field/energy that permeates and surrounds living organisms. However, the energy can exists in several forms such as kinetic, potential, electrical, magnetic, and nuclear. Similarly, the human nervous system consists of the energy and chemical information in the form of electrical signals. Thus, human has the ability to harness the energy from environment or universe and can transmit into any living or nonliving object(s) around the globe. The objects always receive the energy and responding into useful way that is called biofield energy and the process is known as biofield treatment. Few cases of biofield therapies are reported effectively [11,12], but very less controlled and experimental studies on biofield and electromagnetic fields treatment are practiced worldwide [13]. According to law of mass-energy inter-conversion [14], the conversion of mass into energy is well stabilized, but its inversion i.e. energy into mass has not yet proved scientifically. Whenever electrical signals fluctuate with time, the magnetic field generates as per the Ampere-Maxwell law, and cumulatively known as electromagnetic field. Mr. Trivedi’s biofield treatment is well-known to change the physicochemical and atomic characteristics of various materials. Mr. Trivedi’s biofield treatment had been studied and reported in altering the antimicrobial susceptibility and biochemical reactions of microbes against tested antimicrobials [15-17]. It has also significantly reported in field of material science [18-20]. Biofield treated crops had been reported for a significant change on growth, characteristics and yield of plants [21-24]. Present study reports the impact of biofield treatment on MDR isolates of P. aeruginosa, for its antimicrobial susceptibility pattern along with minimum inhibitory concentrations (MIC), biochemical reactions, and biotyping.

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