Physical, Thermal and Spectroscopical Characterization of Biofield Treated Triphenylmethane: An Impact of Biofield Treatment

What is it about?

Triphenylmethane is a synthetic dye used as antimicrobial agent and for the chemical visualization in thin layer chromatography of higher fatty acids, fatty alcohols, and aliphatic amines. The present study was an attempt to investigate the impact of biofield treatment on physical, thermal and spectroscopical charecteristics of triphenylmethane. The study was performed in two groups i.e., control and treatment. The treatment group subjected to Mr. Trivedi’s biofield treatment. The control and treated groups of triphenylmethane samples were characterized using X-ray diffraction (XRD), surface area analyzer, differential scanning calorimetry (DSC), thermogravimetric analysis (TGA), Fourier transform infrared (FT-IR), ultraviolet-visible (UV-Vis) spectroscopy, and gas chromatography-mass spectrometry (GC-MS). XRD study revealed decreases in average crystallite size (14.22%) of treated triphenylmethane as compared to control sample. Surface area analysis showed a slight increase (0.42%) in surface area of treated sample with respect to control. DSC thermogram of treated triphenylmethane showed the slight increase in melting point and latent heat of fusion with respect to control. TGA analysis of control triphenylmethane showed weight loss by 45.99% and treated sample showed weight loss by 64.40%. The Tmax was also decreased by 7.17% in treated sample as compared to control. The FT-IR and UV spectroscopic result showed the similar pattern of spectra. The GC-MS analysis suggested a significant decrease in carbon isotopic abundance (expressed in δ13C, ‰) in treated sample (about 380 to 524‰) as compared to control. Based on these results, it is found that biofield treatment has the impact on physical, thermal and carbon isotopic abundance of treated triphenylmethane with respect to control.

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Why is it important?

Triphenylmethane is a hydrocarbon with molecular formula (C6H5)3CH. It builds the basic skeleton of many synthetic dyes such as bromocresol green, malachite green etc., and used as pH indicator and fluorescence agent [1]. Boulos RA has reported its antimicrobial property [2]. Triphenylmethane reported to inhibit 3-methyl-cholanthrene-induced neoplastic transformation of 10T1/2 cells in a dose-dependent manner and as a novel chemo preventive agent [3]. This has been used as visualizing agentin thin-layer chromatography of higher chain fatty acids, fatty alcohols, and aliphatic amines [4]. Recently, triphenylmethane was reported as an alternative for mediated electronic transfer systems in glucose oxidase biofuel cells (enzymatic biofuel cell). The enzymatic biofuel cell is a type of fuel cell wherein the enzymes are used as a catalyst to oxidize its fuel, instead of costly metals [5]. Hugle et al. used triphenylmethane as a possible moderator material to reduces the speed of neutrons in nuclear chain reactions, especially promising as cold neutrons moderator. It has a unique structure i.e., three aromatic phenyl groups surrounding one central carbon atom that is able to generate a stable radical ion [6]. Diverse applications of triphenylmethane especially as florescent indicator, mediator in biofuel and as a moderator had been suggested the importance of physicochemical property of triphenylmethane. It was previously reported that physical and thermal properties of molecule also affect its reactivity [7,8]. Hence, it is beneficial to find out an alternate approach that can improved the physicochemical properties of compounds like triphenylmethane, which can enhance its usability. Recently, biofield treatment reported to alter the spectral properties of various pharmaceutical drugs like chloramphenicol and tetracycline, and physicochemical properties of metals, beef extract and meat infusion powder [9-11]. Relation between mass and energy (E=mc2) is well reported in literature [12]. The mass (solid matter) is consist of energy and when this energy vibrates at a certain frequency, it provides physical, atomic and structural properties like size, shape, texture, crystal structure, and atomic weight to the matter [13]. Similarly, human body also consists with vibratory energy particles like protons, neutrons, and electrons [14]. Due to vibrations in these particles, an electrical impulse is generated that cumulatively forms electromagnetic field, which is known as biofield [15]. The human has the ability to harness the energy from the environment or Universe and transmit this energy into any object (living or nonliving) on the Globe. The object(s) receive the energy and respond into useful way, this process is known as biofield treatment. Mr. Trivedi’s unique biofield energy is also called as The Trivedi Effect®, and reported to change various physicochemical, thermal and structural properties of several metals [10,16,17] and ceramics [18]. In addition, biofield treatment has been extensively studied in different fields such as agricultural science [19,20], biotechnology research [21], and microbiology research [22-24]. Conceiving the impact of biofield treatment on various living and nonliving things, the study aimed to evaluate the impact of biofield treatment on spectral and physicochemical properties of triphenylmethane using different analytical techniques.

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