LC–MS/MS analysis of lipidized analogs of prolactin-releasing peptide utilizing a monolithic column and simple sample preparation

  • Jana Zemenová, David Sýkora, Anna Freislebenová, Lenka Maletínská
  • Bioanalysis, September 2017, Future Science
  • DOI: 10.4155/bio-2017-0125

LC–MS/MS analysis of lipidized analogs of prolactin-releasing peptide

What is it about?

Obesity is a health issue that has reached epidemic proportions. Novel compounds for obesity treatment are currently being studied employing lipidized analogs of anorexigenic neuropeptides. Various analogs of prolactin-releasing peptide (PrRP) have demonstrated their ability to decrease food intake. However, because of specific properties of lipopeptides it is challenging to find adequate analytical method suitable for their determination in biological samples. We developed and validated a robust LC-MS/MS method for lipidized PrRP analog. The new method was successfully used for in vitro stability study of lipidized PrRP in mice and macaque plasma and the results were compared with data obtained by immonoanalysis.

Why is it important?

The theme is highly relevant as many newly synthesized lipopeptides are being evaluated for various therapeutical targets. Moreover, it seems that standard procedures routinely utilized for sample preparation and peptide analysis might be unsuitable in the case of lipidized peptides.

Perspectives

David Sykora
Vysoka skola chemicko-technologicka v Praze

The application of the developed LC–MS method is envisaged in pharmacokinetic studies of lipopeptides that possess a structure similar to Palm11-PrRP. The goal of such experiments will be to elucidate the mechanism of action and the dosing regimen for the newly synthesized lipopeptides.

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http://dx.doi.org/10.4155/bio-2017-0125

The following have contributed to this page: David Sykora