Sensitive and Comprehensive Analysis of O -Glycosylation in Biotherapeutics: A Case Study of Novel Erythropoiesis Stimulating Protein

What is it about?

Glycosylation of rhEPOs is significantly associated with drug’s quality and potency. Thus, comprehensive characterization of glycosylation is vital to assess the biotherapeutic quality and establish the equivalency of biosimilar rhEPOs. However, current glycan analysis mainly focuses on the N-glycans due to the absence of analytical tools to liberate O-glycans with high sensitivity. We developed selective and sensitive method to profile native O-glycans on rhEPOs.  Typical O-glycans, as well as biologically relevant O-glycan modifications such as sialylation and O-acetylation on rhEPOs, were characterized through O-glycopeptide analyses.

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