What is it about?
This work is a detailed protocol to perform fluorescence staining to visualize intestinal tuft cell using mouse cryosections.
Featured Image
Why is it important?
Lack of reliable antibodies against tuft cell has been a problem to elucidate the physiological role of intestinal tuft cells. Using anti-phospho-girdin antibodies (pY1798), anybody can obtain convincing tuft cell staining results.
Perspectives
Not only its importance as a tuft cell marker, pY1798 gave us another scientific question. Why 'phospho-specific' antibodies visualizes tuft cell so robustly? We may need to revise the idea that tuft cells are originated from a specific lineage.
M.D., Ph. D. Masato Asai
Read the Original
This page is a summary of: Use of Anti-phospho-girdin Antibodies to Visualize Intestinal Tuft Cells in Free-Floating Mouse Jejunum Cryosections, Journal of Visualized Experiments, March 2018, MyJove Corporation,
DOI: 10.3791/57475.
You can read the full text:
Resources
Contributors
The following have contributed to this page
