What is it about?
In this paper we provide very clear and logical instructions on how influenza pseudotypes can be used for serological assays to measure levels of neutralising antibodies in patient serum. These assays can be referred to as pMN (pseudotype Micro-Neutralisation) assays. Pseudotype versions of wildtype viruses are replication-defective and can thus be employed in low-biosafety level laboratories.
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Why is it important?
It is important as it explains in exhaustive detail how to set up a neutralisation assay with pseudotypes, and also how to use GraphPad to accurately calculate the neutralising antibody titre. Info graphics and software screenshots are provided throughout to facilitate ease of use.
Perspectives
Personally I am very proud of this article which was largely put together by my ex PhD student who has co-authored it with me. It is a very useful article to provide to stakeholders for training purposes. It also paves the way for eventual assay standardisation.
Professor Nigel James Temperton
University of Kent
Read the Original
This page is a summary of: Pseudotype Neutralization Assays: From Laboratory Bench to Data Analysis, Methods and Protocols, January 2018, MDPI AG,
DOI: 10.3390/mps1010008.
You can read the full text:
Resources
My lab blog
Influenza pseudotype R&D (Temperton Lab) blog hosted by the University of Kent.
Lab Figshare page
Lab Figshare page with talks, protocols, abstracts and data.
Recommended book chapter
Book chapter entitled "Influenza Virus Titration, Antigenic Characterization, and Serological Methods for Antibody Detection"
Automating pseudotype neutralisation assays with AutoPlate
Demo video on how to use AutoPlate that was co-developed by the University of Cambridge Laboratory of Viral Zoonotics, the Viral Pseudotype Unit in Kent and the company DIOSynVax.
Contributors
The following have contributed to this page
