Functional Impact of the N-terminal Arm of Proline Dehydrogenase from Thermus thermophilus

Mieke Huijbers, Ilona van Alen, Jenny Wu, Arjan Barendregt, Albert Heck, Willem van Berkel
  • Molecules, January 2018, MDPI AG
  • DOI: 10.3390/molecules23010184

The N-terminal arm of proline dehydrogenase

What is it about?

The N-terminal arm of the ubiquitous enzyme proline dehydrogenase (ProDH) differs in length, dependent on the organism. We have studied the functional impact of this arm in a thermophilic ProDH by shortening the arm with deletion mutagenesis.

Why is it important?

Shortening the length of this arm gives insight in the importance of this arm for the catalytic and structural properties of ProDH and provides important information about the aggregation behaviour of ProDH.

Perspectives

Professor Willem J.H. van Berkel
Wageningen University

This study forms a good basis for getting more insight into the evolutionary relationship of ProDH and its interaction with cellular partners.

Read Publication

http://dx.doi.org/10.3390/molecules23010184

The following have contributed to this page: Professor Willem J.H. van Berkel