Characterization of the Filovirus-Resistant Cell Line SH-SY5Y Reveals Redundant Role of Cell Surface Entry Factors

Francisco Zapatero-Belinchón, Erik Dietzel, Olga Dolnik, Katinka Döhner, Rui Costa, Barbara Hertel, Barbora Veselkova, Jared Kirui, Anneke Klintworth, Michael Manns, Stefan Pöhlmann, Thomas Pietschmann, Thomas Krey, Sandra Ciesek, Gisa Gerold, Beate Sodeik, Stephan Becker, Thomas von Hahn
  • Viruses, March 2019, MDPI AG
  • DOI: 10.3390/v11030275

The cell Line SH-SY5Y is resistant to infection by Ebola virus and other filoviruses

What is it about?

The highly pathogenic Ebola virus (EBOV), a member of the family Filoviridae, can enter a diverse range of cell types and only lymphocytes are known to be non-susceptible. This study shows that the cell line SH-SY5Y is resistant to entry of EBOV and other filoviruses and that entry can be restored upon expression of diverse attachment factors.

Why is it important?

This study identifies the first adherent , non-lymphocyte human cell line resistant to filovirus infection. Moreover, it provides the first evidence that the attachment step can limit filovirus entry into target cells.

Perspectives

Professor Stefan Pöhlmann
German Primate Center

The cell line SH-SY5Y will be a great tool to study filovirus interactions with attachment factors (to be stably expressed in this cell line) and it will be interesting to determine whether and how filoviruses can be adapted to efficient entry into these cells.

Read Publication

http://dx.doi.org/10.3390/v11030275

The following have contributed to this page: Professor Stefan Pöhlmann