What is it about?
After BOX-PCR fingerprinting, the genomic sequences of two isolates of S. marcescens were compared to others strains from the NCBI database in other to estimate their evolutionary divergence. The in vitro trypanolytic activity of these two bacterial isolates against Trypanosoma cruzi was assessed by microscopy. In addition, the gene expression of type VI secretion system (T6SS) was detected in vivo by RT-PCR. The in vitro trypanolytic activity of both S. marcescens strains was stronger in their stationary phases of growth than in their exponential ones. Here, we describe their genomic features with the purpose of bringing new insights into the S. marcescens adaptations for colonization of the specific niche of triatomine guts.
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Why is it important?
This study provides the basis for a better understanding of the role of S. marcescens in the microbiota of R. prolixus gut as a potential antagonist of T. cruzi in this complex system.
Perspectives
This publication describes the genomic strucure of two trypanolytic strains of S. marcescens in vivo, which offers the perspective of bacterial shuttle availability for paratrangenesis on a large scale.
Nicolas Carels
Oswaldo Cruz Foundation
Read the Original
This page is a summary of: In vitro Trypanocidal Activity, Genomic Analysis of Isolates, and in vivo Transcription of Type VI Secretion System of Serratia marcescens Belonging to the Microbiota of Rhodnius prolixus Digestive Tract, Frontiers in Microbiology, January 2019, Frontiers,
DOI: 10.3389/fmicb.2018.03205.
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