Expression of the antibody 14D9 in Nicotiana tabacum hairy roots

What is it about?

Nicotiana tabacum hairy roots that express the antibody 14D9 were established. The 14D9 antibody yield obtained after 20 days of culture was 5.95 μg 14D9ml-1. The addition of the reticulum endoplasmic retention sequence KDEL demonstrated a positive effect over the intracellular 14D9 amounts with a yield increase up to 20.82 g ml-1. DMSO increased the antibody amount in the biomass from 20.00 to 64.03 g ml-1 while PVP (at 1.5 gl-1) and gelatine (at 5.0 gl-1) increased total 14D9 amounts in the culture medium to 25 ug and 14 ug respectively.

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Why is it important?

This paper was the first one communicating the expression of the recombinant catalytic antibody 14D9 in hairy roots of tobacco.