What is it about?
Selection of an appropriate collagenase-protease product is critical for recovery of a sufficient number of islets from human pancreata that are required for successful islet transplantation. The review stresses the importance of using intact collagenase in these enzyme mixtures since these forms have the highest activity to degrade native collagen. If collagenase's collagen degradation activity is in excess, then the selection and dose of neutral protease defines the recovery of islets from pancreata.
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Why is it important?
The studies associating the biochemical characteristics of C. histolyticum collagenase to islet yields shows that the highest islet yields are obtained with collagenase-protease enzyme mixtures that have a high proportion of intact collagenase. The narrow specificity of collagenase for native or denatured collagen (i.e., gelatin) shows that excess collagenase activity is not detrimental to cell viability. Therefore, if collagenase is in excess, then cell recovery dependent on the dose and choice of neutral protease used in the isolation procedure.
Perspectives
The conclusions from this review are applicable to any cell isolation procedure that uses collagenase-protease enzymes to recover cells from tissue. A hypothetical model is presented that explains the role collagenase and protease play in degrading the extracellular matrix, leading to the release of cells from the matrix.
Robert McCarthy
VitaCyte
Read the Original
This page is a summary of: Evolution of Enzyme Requirements for Human Islet Isolation, OBM Transplantation, November 2018, Open BioMedical Publishing Corporation,
DOI: 10.21926/obm.transplant.1804024.
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