What is it about?
Rapid growth in genomics along with recombinant DNA (rDNA) technology and gene expression studies stimulated peptide drug research. The promising use of peptide therapeutics demands sensitive and selective quantification methods. Protein sequencing and proteomic investigations can be successfully accomplished through the Mass Spectroscopy (MS) based methods. Mass spectroscopy-based soft ionization methods, namely, electrospray ionization (ESI) and Matrix-Assisted Laser Desorption/Ionization (MALDI), offer high-throughput sequencing to provide the characterization (sequence and structure) of intact proteins/peptides.
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Why is it important?
The advent of tandem Mass Spectrometry (MS/MS) along with data acquisition methods are the basis for the evolution of peptide therapeutics research. The evolution of data science helped in developing computational proteomics, which assists in the quantitative determination of protein samples.
Perspectives
This review narrates the role of mass spectrometry in peptide drug discovery particularly in the sequence characterization along with the latest developments, such as computational proteomics.
Durai Ananda Kumar T
Writing this article was a great pleasure as it has co-authors with whom I have had long standing collaborations. The advent of tandem Mass Spectrometry (MS/MS) along with data acquisition methods are the basis for the evolution of peptide therapeutics research.
Dr Gurubasavaraj V Pujar
JSS college of Pharmacy, JSS University, Mysuru, Karnataka, India 570015
Read the Original
This page is a summary of: Mass Spectrometry (MS/MS) and Proteomics: A High-Performance Bioanalytical Technique for the Peptide Drug Discovery, Mini-Reviews in Organic Chemistry, November 2021, Bentham Science Publishers,
DOI: 10.2174/1570193x17999201029191435.
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