Fluorescence of Glycyl-Tryptophan Peptide on Some Azobenzene Compounds

What is it about?

Five newly synthesized bent-shaped compounds containing azobenzene chromophores have been prepared by resorcinol esterification with 4-(4-alkyloxyphenylazo) benzoic acid using DCC and DMAP. The structure of newly synthesized compounds was confirmed by MS and FT-IR spectroscopy. In addition, NMR spectroscopy also established the purity of these compounds. According to their azo-type chemical structure, the newly synthesized compounds are expected to be highly fluorescent. Consequently, their absorption and fluorescence properties were investigated using UV-Vis and fluorescence spectroscopy. The present paper also investigates the fluorescence evolution of glycyl-L-tryptophan dipeptide at different concentrations of the newly synthesized bent-shaped compounds. The intensity of their fluorescence band over 400 nm depends much on individual structures. Since the investigated compounds could have optical properties characteristic to liquid crystals, the interaction with fluorescent peptides, such as glycyl-tryptophan or proteins might enhance their utility.

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Why is it important?

Although the investigated compounds display an intense absorption in the UV range at about 370 nm, the intensity depends much on structure of the terminal chain. Besides, they have a fluorescence band over 400 nm, whose intensity also depends on each compound structure. For example, Rez10S had an intense absorption in the UV range and two maxima of fluorescence at 410 nm and 735 nm. Moreover, the investigated compounds induced a very specific fluorescence of peptide GW at 556 nm.

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