What is it about?
PDE12 or 2´-PDE is an endogeous intracellular enzyme that degrades 2´-5´oligoadenylates, signalling molecules that if present mediate an ani-viral state via activation of RNase L and subsequent Degradation of cellular and viral RNA. After Inhibition of PDE12 an increase in 2´-5´oligoadenylates is expected to result in a more pronounced anti-viral state of the treated cells.
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Why is it important?
Several viruses (including the human coronavirus MERS-CoV, PMID: 27025250 ) have been published to code for proteins with PDE12 - like activity. This is believed to result in the down-regulation of the cellular defense against virus infection / production of new viral particles. If used together with recombinant viral proteins with PDE-12 activity, the described screening assay could possibly be used to identify selective compound-inhibitors of this viral activity, that might improve cellular virus defense.
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This page is a summary of: Development of a Scintillation Proximity Assay (SPA) Based, High Throughput Screening Feasible Method for the Identification of PDE12 Activity Modulators, Current Drug Discovery Technologies, October 2016, Bentham Science Publishers,
DOI: 10.2174/1570163813666160623094451.
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