Differentiation of amyloid and non-amyloid type aggregates using flow cytometry (FACS)

What is it about?

Protein aggregates are of two types. Amyloid types and non-amyloid types. Depending on their size and granularity they can be distinguished using laser based flow cytometric analysis.

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Why is it important?

There are various methods available for distinguishing the amyloid and non-amyloid types of aggregates including electrochemistry, chromatography, electron microscopy etc. But the flow cytometry based analysis exploits the inherent properties of the aggregates like size and the granularity of the aggregates, therefore, this method is a direct method of analysis where some of the available reported methods are indirect methods.