What is it about?

New analytical method development or optimization of existing methods have to be carried out according to the validation guidelines of ICH Q2(R1). These steps are essential for drug development, new formulation development, and maintaining the quality attribute of pharmaceutical formulations. It also assures the scientific integrity of analytical results and provides documented evidence of method performance. Recently, green analytical methods have been developed using greener solvents (s) and reagents because of the increasing impact of toxic organic solvents on the environment, and analysts. This article reviewed various modern analytical techniques, including UV-Vis. spectroscopy, HPLC, HPTLC, LC-MS/MS, GC-MS, electrochemical method, and Spectrofluorimetry. It also includes a few green analytical methods for HPLC and HPTLC. Assessment of reviewed analytical methods is done in the Section 2, where analysis of the pharmaceutical formulation matrix was mainly done by UV-Vis. spectroscopy and HPLC technique. The LC-MS technique is mainly used for bioanalysis, which involves pharmacokinetic study and bioequivalence study. Sensitive HPLC methods can also be used for the analysis of biological matrices. Electrochemical processes should be used for fast, easy handling and avoiding extra physical and chemical steps in estimating drugs. This review article provides feedback on various analytical methods so that researchers and analysts can revise, improve the existing techniques, and develop a new strategy.

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Why is it important?

Our findings help the analyst to develop a new analytical method with a short period of time.


Writing this article was a great pleasure as it has co-authors with whom I have had long standing collaborations. This article also lead to help the researchers for developing a new analytical method.


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This page is a summary of: Application of Modern Analytical Techniques for Quantification of Tadalafil in Pharmaceutical and Biological Matrices: Future Prospectus, Current Pharmaceutical Analysis, February 2023, Bentham Science Publishers,
DOI: 10.2174/1573412919666221115123010.
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