What is it about?
Compared with the traditional cumbersome non-homologous recombinant inhibitor screening process, the improved zebrafish embryo survival rate was used as an indicator to simplify the drug screening process.
Featured Image
Why is it important?
First, the phenomenon of non-homologous recombination is significant during the development of zebrafish embryos. Second, the role of non-homologous recombinant inhibitors in tumor therapy and postoperative prevention has been paid more and more attention. Combining these two points, zebrafish embryo model can be a simple and high throughput non-homologous recombinant inhibitor drug screening model.
Perspectives
This article is an accidental discovery in my experiment. Zebrafish embryos showed very high mortality rates due to injection of SV40 abort fragments. Validation of Southern blot confirmed that the injected SV40 fragments were integrated into the embryonic genome. The non homologous recombination of zebrafish embryos during early development is combined with tumor therapy. By adding non-homologous recombinant inhibitors, zebrafish embryo survival rate can be effectively improved, which can be used as an indicator for functional identification of non-homologous recombinant inhibitors.
Zhe Yang
Yangzhou University
Read the Original
This page is a summary of: Injection of an SV40 transcriptional terminator causes embryonic lethality: a possible zebrafish model for screening nonhomologous end-joining inhibitors, OncoTargets and Therapy, August 2018, Dove Medical Press,
DOI: 10.2147/ott.s153576.
You can read the full text:
Contributors
The following have contributed to this page
