What is it about?
It remains unsettled questions what is the difference between invaded (responders) and non-invaded cells (non-responders) against invasion-inducible stimuli. However, none of cancer invasion models can capture separately invaded and non-invaded cells at the end of experiments as living cells.
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Why is it important?
We developed a novel invasion assay that enables to catch and analyze both invaded and non-invaded cells separately. The apparatus had two gel layers separated by a nylon mesh membrane with 40-µm2 pores that were large enough to allow passage of invading cells. For instance, cell invasion from Matrigel to collagen gel can be analyzed.
Perspectives
Invasive and non-invasive cell populations can be further enriched by repeating the iCT assay and may be used for transcriptome, proteome, and genome analyses.
Takino Takahisa
Read the Original
This page is a summary of: Simple and cost-effective assay for isolating invasive living cells, BioTechniques, September 2018, Future Science,
DOI: 10.2144/btn-2018-0036.
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