UJI AKTIVITAS ANTIBAKTERI EKSTRAK METANOL MIKROALGA Chlorella sp. PADA FASE STASIONER HASIL KULTIVASI DALAM MEDIUM EKSTRAK TAUGE (MET)

What is it about?

Chlorella sp., belonging to Chlorophyta, contains several important compound such as flavonoid, tanin, phenolic compound, terpenoid, chlorophyll and karotenoid. This study was aimed to determine antibacterial activity from methanol extract of Chlorella sp. at stationary phase of its growth and to identify the active compound group in the extract of Chlorella sp. Chlorella sp. was cultivated in 4 % of Tauge Extract Medium (TEM). The extraction of microalgae Chlorella sp. was performed by maceration with methanol solvent. The antibacterial activities of methanol extract from stationary phase were assayed using diffusion method toward E. coli and Staphylococcus aureus with different concentration of extract (5 %, 10 %, 15 %, 20 %, and 25 %). Identification of active compound was estimated by reagent tested on qualitative scale include alkaloid, falvonoid, steroid, triterpenoid, and tanin. The result showed that methanol extract of Chlorella sp. in stationary phase in different concentration could inhibit the growth of E. coli and S. aureus bacterium. Chlorella sp. also contains a steroid and tanin compound class.

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