What is it about?
Abstract: Due to the overuse and misuse of antibiotic, an increase in antibiotic resistance of pathogenic bacteria is evolving. Attention should be focused on natural alternatives to antibiotics, like propolis, royal jelly (RJ) and honeys. They all have strong antibacterial properties due to the active substances they contain. This study investigated the effect of combination of water soluble propolis (WSP) Greit120 or fresh royal jelly (F-RJ) (Mižigoj) and Forest honeys as antibacterial against Escherichia coli, Pseudomonas aeruginosa, Proteus mirabilis, Acinetobacter baumanii, Staphylococcus aureus, methicillin resistant Staphylococcus aureus (MRSA), Streptococcus pyogenes, Streptococcus agalactiae and Candida albicans. These substances are also cell growth promoters for human macrophage (TLT) cell line. WSP Greit120, F-RJ (M) and different Forest honeys were prepared in saline as 10% solutions. The antimicrobial activity was expressed as the minimal inhibitory concentration (MIC) in mg/mL. The growth promotion activity was measured at optical density (OD) 595 nm. The combination of WSP Greit120 with different Forest honeys is better than F-RJ (M) in same combination with different Forest honeys. The best antibacterial/antifungal activity was found with the combination of 10% WSP Greit120 in the Forest honey (1:10) from Italy or Spain. When measuring the growth promoting activity of TLT cell line, the best activity was detected at the combination of 10% WSP Greit120 in the Forest honey from Italy (GI3 = 0.796 ± 0.014 and GI5 = 1.133 ± 0.022). Antimicrobial and growth promoting activities are correlated and WSP-dependent.
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Why is it important?
The present experiments were aimed, at very first time, to compare the enhancement of different Forest honey’s antimicrobial activity in combination with water soluble propolis (WPS) Greit120 or fresh royal jelly (F-RJ) (Mižigoj). In addition, these combinations were analysed for growth enhancing activity of human macrophage (TLT) cell line in vitro as a possible model of wound healing.
Perspectives
The F-RJ (M) combined with the Bio-Forest honey (Italy), Forest Honey (Italy) or Forest honey from Spain enhances antibacterial activity against Gram-positive bacteria with fold decrease from median MIC for MRSA (1×), S. aureus (8×), S. pyogenes (1×) and S. agalactiae (1×). For Gram-negative bacteria, the fold decrease from median MIC were found for E. coli (2×), P. aeruginosa (2×), P. mirabilis (4×), A. baumanii (1×) and yeast C. albicans (1×). Unfortunately decrease from median MIC was not statistical significant (t test). The WSP Greit120 somehow enhances the Forest honey’s antimicrobial activity against Gram-positive bacteria with fold decrease from median MIC of Forest honeys for MRSA (8×), S. aureus (2×), S. pyogenes (8×), and S. agalactiae (4×). When Gram-negative bacteria were analysed, the fold decrease from median MIC of Forest honeys were found for E. coli (8×), P. aeruginosa (16×), P. mirabilis (8×), A. baumanii (2×) and yeast C. albicans (16×). The WSP Greit120 in combination with Forest honey on other site stimulates the monocytes/macrophages, according to the data of TLT cells, to release cytokines important for wound healing, together with the activation of the macrophage and neutrophils and protecting the healing tissue from pathogenic infection.
Dr. Bratko Filipič
Univerza v Ljubljani
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This page is a summary of: Water Soluble Propolis and Royal Jelly Enhance the Antimicrobial Activity of Honeys and Promote the Growth of Human Macrophage Cell Line, Journal of Agricultural Science and Technology B, January 2016, David Publishing Company,
DOI: 10.17265/2161-6264/2016.01.005.
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