What is it about?
[Mg2+]o/[Ca2+]o of the culture media determines the number, duration and frequency of the Ca2+oscillations. We found that the Time Incrementation between Oscillations (TIbO) gauges the very early functional impact of culture media and makes it possible to establishe possible correlations with adult phenotypes.
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Why is it important?
The finding of TIbO coefficient provides a means to quantify and compare the functional impact of different IVF media used for animal and human eggs. The results suggest that an accumulative blockage of Ca2+influx, named ABCI, constantly delay the occurrence of the next oscillation. The fidelity of this phenomenon whatever the variability of the Ca2+ response, make the frequency, the duration and the total numbers of Ca2+ oscillations predictable once the oocyte have been fertilized. This mechanism offers a physiological mean to tune at will the number of Ca2+ oscillations by varying external [Mg2+]o/[Ca2+]o ratio in media during egg activation.
Perspectives
It has been always difficult to correlate Ca2+ oscillations with observable post-natal consequences due to the use of technical or chemical artifacts that jeopardize the interpretation or the results. The high sensitivity of the Ca2+ oscillations to external Mg2+ /Ca2+ ratio offers new opportunities to control the Ca2+ regime. However, it remains to design specific media formulations that induce 5, 10, 20 or more oscillations on purpose with high reliability and low variability and record the developmental outcome (without the need of Ca2+ recording). The use of algorithm for Ca2+ regime analysis makes it possible to establish empirical models which help designing new media formulation based on functional data.
Jean-Pierre Ozil
INRA France
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[Mg
2+
]
o
/[Ca
2+
]
o
determines Ca
2+
response at fertilization: tuning of adult phenotype?
, Reproduction, August 2017, Bioscientifica,
DOI: 10.1530/rep-16-0057.
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