Effect of high salt stress on mungbean (Vigna radiata) seedling cells

What is it about?

The high salt stress to the seedling is the substantial ecological problem in the ongoing era. It negatively influences the growth which retard mitotic division by enhancing aberrations in nuclear chromatin. In the light of these views, the current work was designed to investigate the response of mungbean (Vigna radiata) seedlings root tip cells to the presence of NaCl ions. The seeds of NM-92 and NM19-19 varieties of mungbean were imbibed separately in distilled water for 24 hours and allowed to grow into 0, 50, 150, 250, and 350 mM NaCl solution for 24 hours. An excised root tips were stained, and slides were scored at 100x objective for the mitotic index (MI) and chromosomal aberrations. The data demonstrated that as NaCl concentration increased, the MI was declined along with various chromatin abnormalities. The 150 mM of NaCl showed more lagging (69%) of chromosomes during anaphase in NM19-19. The highest stickiness at metaphase stage (68%) was found in 250 mM NaCl in variety NM19-19. However, both varieties were differed non-significantly for c-mitosis that was recorded 99% at 350 mM NaCl concentration. It was concluded that the NaCl ions toxicity induced various cytological anomalies in seedling roots that adversely affect the growth of mungbean seedlings.

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