New simple multiplex Real-Time PCR assays for foodborne pathogens detection on LightCycler.

What is it about?

Most of the acute intestinal diseases are caused by food-borne pathogens. The aim of this study was to develop a real-time PCR-based fast and low-cost procedure to simultaneously detect contamination of food by any of five food-borne pathogens: Campylobacter jejuni, Mycobacterium bovis, Enterobacter sakazaki, Shigella boydii, Clostridium perfrigens using multiplex EvaGreen real-time PCR. The real-time PCR assay described in this study has the potential to be a fast screening assay for several pathogens enabling simultaneous processing of many samples.

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Why is it important?

This assay may be used for accurate and rapid diagnosis of food-borne outbreaks, it has the potential to be used in routine diagnostic laboratories providing a fast, cheap and sensitive alternative method to culture-based or TaqMan qPCR methods.