What is it about?
A sensitive method for cyclophosphamide determination in urine with high recovery was developed and validated for monitoring occupational exposure. Triple liquid-liquid extraction with ethyl acetate/dichloromethane (φr = 3 : 1; 6.0 mL) was applied. Good separation of cyclophosphamide and ifosfamide was achieved in 9.0 min in liquid chromatography combined with tandem mass spectrometry (LC-MS/MS). Concentrations as 0.07 ng mL−1 could be detected successfully usin this method. Mean recovery was 101.62%.
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Why is it important?
Pharmacy staff and health care workers in hospitals may be exposed to antineoplastic drugs during cancer chemotherapy. Sensitive methods should be used to monitor the occupational exposure in biofluids such as urine, because the exposure amounts appear in urine are usually extremely low to detect .
Perspectives
Since very low concentrations could be observed with this method , it is very suitable to monitor the occupational exposure in urine. The method was prooved to work in real samples. The real sample results obtained from various hospitals are not given here, which was the subject of a dissertation thesis of one of the authors of this study.
Assoc. Prof. Dr. Beril ANILANMERT
Istanbul Üniversitesi-Cerrahpaşa
Read the Original
This page is a summary of: Validated method for occupational cyclophosphamide monitoring using LC-MS/MS and a Poroshell 120 column, Chemical Papers, January 2015, Springer Science + Business Media,
DOI: 10.1515/chempap-2015-0157.
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