What is it about?
CRISPRs are regions of prokaryotic DNA containing short, repetitive sequences. Each repetition is flanked by short segments of spacer DNA originated from previous exposures to foreign DNA. In bioengineering, CRISPR/Cas9 has been applied to edit genomes. By delivering the Cas9 nuclease and synthetic guide RNA (gRNA) into a cell to cut a genome at a desired location, allowing existing genes to be excised and/or new ones added.
Featured Image
Why is it important?
The review covers very briefly original papers about the CRISPR in prokaryotic cells and fast development of the CRISPR/Cas based technology to modify eukaryotic genomes.
Perspectives
The paper serves to readers of non-biology or non-genetics backgrounds to introduce them to one of the fastest developing biotech tools in recent years.
svetlana kryštofová
Slovenska Technicka Univerzita
Read the Original
This page is a summary of: CRISPR/Cas in genome defense and gene editing, Acta Chimica Slovaca, January 2016, De Gruyter,
DOI: 10.1515/acs-2016-0012.
You can read the full text:
Contributors
The following have contributed to this page
